ZUBAL, L., W. BONANI, D. MANIGLIO, R. CECCATO, D. RENCIUK, Aleš HAMPL, C. MIGLIARESI, J. JANCAR and L. VOJTOVA. Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions. Online. eXPRESS Polymer Letters. Budapest: Budapest University of Technology and Economics, Department of Polymer Engineering, 2018, vol. 12, No 2, p. 159-170. ISSN 1788-618X. Available from: https://dx.doi.org/10.3144/expresspolymlett.2018.14. [citováno 2024-04-24]
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Basic information
Original name Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions
Authors ZUBAL, L. (203 Czech Republic, guarantor), W. BONANI (380 Italy), D. MANIGLIO (380 Italy), R. CECCATO (380 Italy), D. RENCIUK (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution), C. MIGLIARESI (380 Italy), J. JANCAR (203 Czech Republic) and L. VOJTOVA (203 Czech Republic)
Edition eXPRESS Polymer Letters, Budapest, Budapest University of Technology and Economics, Department of Polymer Engineering, 2018, 1788-618X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10404 Polymer science
Country of publisher Hungary
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.875
RIV identification code RIV/00216224:14110/18:00102232
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3144/expresspolymlett.2018.14
UT WoS 000419153200006
Keywords in English biocompatible polymers; collagen fibrillogenesis; structural analysis; viscoelastic properties; protein self-assembly
Tags 14110517, EL OK, rivok
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 10/2/2019 19:43.
Abstract
Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.
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