Genetic predisposition for chronic venous insufficiency in
several genes for matrix metalloproteinases (MMP-2, MMP-9,
MMP-12) and their inhibitor TIMP-2

J 2017

Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2

SLONKOVÁ, Veronika, Veronika Jr. SLONKOVÁ, Anna VAŠKŮ and Vladimír VAŠKŮ

Basic information

Original name

Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2

Authors

SLONKOVÁ, Veronika (203 Czech Republic, guarantor, belonging to the institution), Veronika Jr. SLONKOVÁ (203 Czech Republic), Anna VAŠKŮ (203 Czech Republic, belonging to the institution) and Vladimír VAŠKŮ (203 Czech Republic, belonging to the institution)

Edition

Journal of the European Academy of Dermatovenereology, Hoboken, Wiley-Blackwell, 2017, 0926-9959

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30216 Dermatology and venereal diseases

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.287

RIV identification code

RIV/00216224:14110/17:00099676

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/jdv.14447

UT WoS

000413582400055

Keywords (in Czech)

venózní insuficience, MMP; TIMP

Keywords in English

Venous insufficiency; MMP; TIMP

Abstract

V originále

OBJECTIVE: The project was scheduled as a case-control study to investigate the correlation between MMP-2 (rs243864), MMP-9 (3918242), MMP-12 (rs7123600) and TIMP-2 (rs8176329) polymorphisms and chronic venous disease (CVD) risk. The genotype and phenotype research envisages the testing of possible associations between MMP and TIMP-2 genotypes and phenotypes of CVD. MATERIAL AND METHODS: 150 patients with CVD and 227 controls were enrolled into the study. The MMPs and TIMP-2 genotypes were identified by the PCR method and restriction analysis according to standard protocols. RESULTS: The G allele of MMP-2 -790 T/G was 1.85 times more frequent in men with CVD than in the control group (P = 0.008). The T allele of MMP-9 -1562 C/T was observed 2.571 times more frequently in patients with CVD than in the control individuals (both in men and women) with clinically significant specificity (P = 0.0000009). The G allele of MMP-12 rs7123600 was determined 2.082 times more frequently in female patients with CVD than in the control group with clinically significant specificity (P = 0.02). No significant result in TIMP-2 rs8176329 polymorphism in the case-control study was observed. CVD women with G allele in MMP-2 -790 T/G in the genotype-phenotype study are seen to develop ulceration 2.539 times more frequently (P = 0.003). The G allele of MMP-12 rs7123600 was detected 3.167 times more frequently in CVD women with ulceration compared with CVD women without ulceration (P = 0.007). In CVD men in C6 stage, the incidence of AG genotype in rs7123600 MMP-12 polymorphism was found to be 4.675 times higher compared to CVD women with C6 staging (P = 0.005). The AG genotype in TIMP 2 rs8176329 polymorphism was found to be associated with higher risk of tumour (P = 0.01). CONCLUSION: Studying these polymorphisms can contribute to better identification of patients at higher risk of developing CVD, while providing the most appropriate prevention and treatment strategies.

Links

MUNI/A/1401/2016, interní kód MU

Name: Patofyziologické biomarkery u komplexních nemocí (Acronym: Biomarkery)

Investor: Masaryk University, Category A

MUNI/A/1420/2016, interní kód MU

Name: Hodnocení diagnostických a terapeutických metod u pacientů s vybranými kožními nemocemi (Acronym: Genetika, světloléčba)

Investor: Masaryk University, Category A

Citovat

SLONKOVÁ, Veronika, Veronika Jr. SLONKOVÁ, Anna VAŠKŮ and Vladimír VAŠKŮ. Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2. Journal of the European Academy of Dermatovenereology. Hoboken: Wiley-Blackwell, 2017, vol. 31, No 10, p. 1746-1752. ISSN 0926-9959. Available from: https://dx.doi.org/10.1111/jdv.14447.

@article{1405510,
   author = {Slonková, Veronika and Slonková, Veronika Jr. and Vašků, Anna and Vašků, Vladimír},
   article_location = {Hoboken},
   article_number = {10},
   doi = {http://dx.doi.org/10.1111/jdv.14447},
   keywords = {Venous insufficiency; MMP; TIMP},
   language = {eng},
   issn = {0926-9959},
   journal = {Journal of the European Academy of Dermatovenereology},
   title = {Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2},
   volume = {31},
   year = {2017}
}

TY  - JOUR
ID  - 1405510
AU  - Slonková, Veronika - Slonková, Veronika Jr. - Vašků, Anna - Vašků, Vladimír
PY  - 2017
TI  - Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2
JF  - Journal of the European Academy of Dermatovenereology
VL  - 31
IS  - 10
SP  - 1746-1752
EP  - 1746-1752
PB  - Wiley-Blackwell
SN  - 09269959
KW  - Venous insufficiency
KW  - MMP
KW  - TIMP
N2  - OBJECTIVE: The project was scheduled as a case-control study to investigate the correlation between MMP-2 (rs243864), MMP-9 (3918242), MMP-12 (rs7123600) and TIMP-2 (rs8176329) polymorphisms and chronic venous disease (CVD) risk. The genotype and phenotype research envisages the testing of possible associations between MMP and TIMP-2 genotypes and phenotypes of CVD. MATERIAL AND METHODS: 150 patients with CVD and 227 controls were enrolled into the study. The MMPs and TIMP-2 genotypes were identified by the PCR method and restriction analysis according to standard protocols. RESULTS: The G allele of MMP-2 -790 T/G was 1.85 times more frequent in men with CVD than in the control group (P = 0.008). The T allele of MMP-9 -1562 C/T was observed 2.571 times more frequently in patients with CVD than in the control individuals (both in men and women) with clinically significant specificity (P = 0.0000009). The G allele of MMP-12 rs7123600 was determined 2.082 times more frequently in female patients with CVD than in the control group with clinically significant specificity (P = 0.02). No significant result in TIMP-2 rs8176329 polymorphism in the case-control study was observed. CVD women with G allele in MMP-2 -790 T/G in the genotype-phenotype study are seen to develop ulceration 2.539 times more frequently (P = 0.003). The G allele of MMP-12 rs7123600 was detected 3.167 times more frequently in CVD women with ulceration compared with CVD women without ulceration (P = 0.007). In CVD men in C6 stage, the incidence of AG genotype in rs7123600 MMP-12 polymorphism was found to be 4.675 times higher compared to CVD women with C6 staging (P = 0.005). The AG genotype in TIMP 2 rs8176329 polymorphism was found to be associated with higher risk of tumour (P = 0.01). CONCLUSION: Studying these polymorphisms can contribute to better identification of patients at higher risk of developing CVD, while providing the most appropriate prevention and treatment strategies.
ER  -

SLONKOVÁ, Veronika, Veronika Jr. SLONKOVÁ, Anna VAŠKŮ and Vladimír VAŠKŮ. Genetic predisposition for chronic venous insufficiency in several genes for matrix metalloproteinases (MMP-2, MMP-9, MMP-12) and their inhibitor TIMP-2. \textit{Journal of the European Academy of Dermatovenereology}. Hoboken: Wiley-Blackwell, 2017, vol.~31, No~10, p.~1746-1752. ISSN~0926-9959. Available from: https://dx.doi.org/10.1111/jdv.14447.

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