Simultaneous analysis of cardiomyocyte contractions by Atomic
force microscopy and calcium imaging

a 2017

Simultaneous analysis of cardiomyocyte contractions by Atomic force microscopy and calcium imaging

PEŠL, Martin, Guido CALUORI, Jan PŘIBYL, Vratislav CMIEL, Tomas POTOCNAK et. al.

Basic information

Original name

Simultaneous analysis of cardiomyocyte contractions by Atomic force microscopy and calcium imaging

Authors

PEŠL, Martin, Guido CALUORI, Jan PŘIBYL, Vratislav CMIEL, Tomas POTOCNAK, Petr SKLÁDAL, Šárka JELÍNKOVÁ, Vladimír ROTREKL and Ivo PROVAZNÍK

Edition

AFM Biomed Conference, Krakow 2017, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Cardiovascular diseases including cardio-surgery

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Abstract

V originále

The study of dynamic Ca2+changes in in vitro models of cardiomyocytes has become a cornerstone to understand the role of calcium signaling in healthy and diseased hearts1 . Atomic force microscopy (AFM) is a highlysensitive and versatile method that can be integrated with optical microscopy and calcium imaging2 . We have implemented a combined set up to measure contractility and calcium waves in human cardiac models. We successfully report the first simultaneous recording of cardiac contractility, though AFM mechanocardiograms (MCG)3 and local calcium waves probed on embryoid bodies. Ensemble empirical mode decomposition (EEMD) filtering was proved the best in terms of signal-to-noise ratio and signal distortions. Caffeine stimulation confirmed the detection capabilities of the used algorithms, measuring the expected physiological response (e.g. calcium duration increased 5.5% and contraction increased 14.5%). The combination of AFM and calcium imaging allows accurate analysis of complex excitation contraction coupling during physiological, disease and drug-induced situations.

Links

MUNI/A/1010/2016, interní kód MU

Name: Efekt elektroporační ablace na lidské srdeční buňky

Investor: Masaryk University, Category A

Citovat

PEŠL, Martin, Guido CALUORI, Jan PŘIBYL, Vratislav CMIEL, Tomas POTOCNAK, Petr SKLÁDAL, Šárka JELÍNKOVÁ, Vladimír ROTREKL and Ivo PROVAZNÍK. Simultaneous analysis of cardiomyocyte contractions by Atomic force microscopy and calcium imaging. In AFM Biomed Conference, Krakow 2017. 2017.

@proceedings{1405779,
   author = {Pešl, Martin and Caluori, Guido and Přibyl, Jan and Cmiel, Vratislav and Potocnak, Tomas and Skládal, Petr and Jelínková, Šárka and Rotrekl, Vladimír and Provazník, Ivo},
   booktitle = {AFM Biomed Conference, Krakow 2017},
   language = {eng},
   title = {Simultaneous analysis of cardiomyocyte contractions by Atomic force microscopy and calcium imaging},
   year = {2017}
}

TY  - CONF
ID  - 1405779
AU  - Pešl, Martin - Caluori, Guido - Přibyl, Jan - Cmiel, Vratislav - Potocnak, Tomas - Skládal, Petr - Jelínková, Šárka - Rotrekl, Vladimír - Provazník, Ivo
PY  - 2017
TI  - Simultaneous analysis of cardiomyocyte contractions by Atomic force microscopy and calcium imaging
N2  - The study of dynamic Ca2+changes in in vitro models of cardiomyocytes has become a cornerstone to understand the role of calcium signaling in healthy and diseased hearts1 . Atomic force microscopy (AFM) is a highlysensitive and versatile method that can be integrated with optical microscopy and calcium imaging2 . We have implemented a combined set up to measure contractility and calcium waves in human cardiac models. We successfully report the first simultaneous recording of cardiac contractility, though AFM mechanocardiograms (MCG)3 and local calcium waves probed on embryoid bodies. Ensemble empirical mode decomposition (EEMD) filtering was proved the best in terms of signal-to-noise ratio and signal distortions. Caffeine stimulation confirmed the detection capabilities of the used algorithms, measuring the expected physiological response (e.g. calcium duration increased 5.5% and contraction increased 14.5%). The combination of AFM and calcium imaging allows accurate analysis of complex excitation contraction coupling during physiological, disease and drug-induced situations.
ER  -

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