Comparative cell cycle transcriptomics reveals synchronization
of developmental transcription factor networks in cancer cells

J 2017

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

BOSTROM, Johan, Zuzana ŠRÁMKOVÁ, Alena SALAŠOVÁ, Helena Anna Dagmar JOHARD, Diana MAHDESSIAN et. al.

Basic information

Original name

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Authors

BOSTROM, Johan (752 Sweden), Zuzana ŠRÁMKOVÁ (703 Slovakia, belonging to the institution), Alena SALAŠOVÁ (203 Czech Republic), Helena Anna Dagmar JOHARD (752 Sweden, belonging to the institution), Diana MAHDESSIAN (752 Sweden), Radek FEDR (203 Czech Republic, belonging to the institution), Carolyn MARKS (752 Sweden), Jiřina MEDALOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic), Emma LUNDBERG (752 Sweden), Sten LINNARSON (752 Sweden), Vítězslav BRYJA (203 Czech Republic), Petra SEKYROVÁ (203 Czech Republic, belonging to the institution), Mikael ALTUN (752 Sweden) and Michael ANDÄNG (752 Sweden, guarantor, belonging to the institution)

Edition

PLOS ONE, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14740/17:00095459

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0188772

UT WoS

000417648600013

Keywords in English

cell cycle; synchronization; fucci reporter

Abstract

V originále

The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.

Links

EE2.3.20.0180, research and development project

Name: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny

GA15-11707S, research and development project

Name: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Czech Science Foundation

GA15-20818S, research and development project

Name: Cílená terapie proti rezistentím kmenovým buňkám gliomového nádoru - kontrola přísunu živin pomocí iontových kanálů

Investor: Czech Science Foundation

GA15-21789S, research and development project

Name: Dishevelled - identifikace základních koordinát

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV15-33999A, research and development project

Name: Vývoj nových nízkomolekulárních protinádorových léčiv na principu syntetické letality

Citovat

BOSTROM, Johan, Zuzana ŠRÁMKOVÁ, Alena SALAŠOVÁ, Helena Anna Dagmar JOHARD, Diana MAHDESSIAN, Radek FEDR, Carolyn MARKS, Jiřina MEDALOVÁ, Karel SOUČEK, Emma LUNDBERG, Sten LINNARSON, Vítězslav BRYJA, Petra SEKYROVÁ, Mikael ALTUN and Michael ANDÄNG. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. PLOS ONE. Public Library of Science, 2017, vol. 12, No 12, p. 0188772-188795. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0188772.

@article{1405981,
   author = {Bostrom, Johan and Šrámková, Zuzana and Salašová, Alena and Johard, Helena Anna Dagmar and Mahdessian, Diana and Fedr, Radek and Marks, Carolyn and Medalová, Jiřina and Souček, Karel and Lundberg, Emma and Linnarson, Sten and Bryja, Vítězslav and Sekyrová, Petra and Altun, Mikael and Andäng, Michael},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pone.0188772},
   keywords = {cell cycle; synchronization; fucci reporter},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1405981
AU  - Bostrom, Johan - Šrámková, Zuzana - Salašová, Alena - Johard, Helena Anna Dagmar - Mahdessian, Diana - Fedr, Radek - Marks, Carolyn - Medalová, Jiřina - Souček, Karel - Lundberg, Emma - Linnarson, Sten - Bryja, Vítězslav - Sekyrová, Petra - Altun, Mikael - Andäng, Michael
PY  - 2017
TI  - Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells
JF  - PLOS ONE
VL  - 12
IS  - 12
SP  - 0188772
EP  - 0188772
PB  - Public Library of Science
SN  - 19326203
KW  - cell cycle
KW  - synchronization
KW  - fucci reporter
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724894/pdf/pone.0188772.pdf
N2  - The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.
ER  -

BOSTROM, Johan, Zuzana ŠRÁMKOVÁ, Alena SALAŠOVÁ, Helena Anna Dagmar JOHARD, Diana MAHDESSIAN, Radek FEDR, Carolyn MARKS, Jiřina MEDALOVÁ, Karel SOUČEK, Emma LUNDBERG, Sten LINNARSON, Vítězslav BRYJA, Petra SEKYROVÁ, Mikael ALTUN and Michael ANDÄNG. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. \textit{PLOS ONE}. Public Library of Science, 2017, vol.~12, No~12, p.~0188772-188795. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0188772.

Detailed Information on Publication Record