Structure and Dynamics of Alginate Gels Cross-Linked by
Polyvalent Ions Probed via Solid State NMR Spectroscopy

J 2017

Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy

BRUS, Jiří, Martina URBANOVA, Jiří CZERNEK, Miroslava PAVELKOVÁ, Kateřina KUBOVÁ et. al.

Basic information

Original name

Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy

Authors

BRUS, Jiří (203 Czech Republic), Martina URBANOVA (203 Czech Republic), Jiří CZERNEK (203 Czech Republic), Miroslava PAVELKOVÁ (203 Czech Republic), Kateřina KUBOVÁ (203 Czech Republic), Jakub VYSLOUŽIL (203 Czech Republic), Sabina ABBRENT (203 Czech Republic), Rafal KONEFAL (203 Czech Republic), Jiří HORSKY (203 Czech Republic), David VETCHÝ (203 Czech Republic), Jan VYSLOUŽIL (203 Czech Republic, belonging to the institution) and Pavel KULICH (203 Czech Republic)

Edition

Biomacromolecules, Washington, American Chemical Society, 2017, 1525-7797

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 5.738

RIV identification code

RIV/00216224:14310/17:00120342

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.biomac.7b00627

UT WoS

000407869400026

Keywords in English

NUCLEAR-MAGNETIC-RESONANCE; EGG-BOX MODEL; SEGMENTAL DYNAMICS; DIVALENT-CATIONS; CALCIUM-ALGINATE; SODIUM ALGINATE; ACID; DELIVERY; COMPLEXATION; COMBINATION

Abstract

V originále

Alginate gels are an outstanding biomaterial widely applicable in tissue engineering, medicine, and pharmacy agent delivery, respectively. This contribution provides new and comrehensive into the atomic-resolution structure for cell transplantation, wound healing and efficient bioactive agent delivery, respectively. This contribution provides new and comrehensive insight into the atomic-resolution structure and dynamics of polyvalent ion-cross-linked alginate gels in microbead formulations. By applying various advanced solid-state NMR (ssNMR) spectroscopy techniques, we verified the homogeneous distribution of the cross-linking ions in the alginate gels and the high degree of ion exchange. We also established that the two-component character of the alginate gels arises from the concentration fluctuations of residual water molecules that are preferentially localized along polymer chains containing abundant mannuronic acid (M) residues. These hydrated M-rich blocks tend to self-aggregate into subnanometer domains. The resulting coexistence of two types of alginate chains differing in segmental dynamics was revealed by H-1-C-13 dipolar profile analysis, which indicated that the average fluctuation angles of the stiff and mobile alginate segments were about 5-9 degrees or 30 degrees, respectively. Next, the C-13 CP/MAS NMR spectra indicated that the alginate polymer microstructure was strongly dependent on the type of cross-linking ion. The polymer chain regularity was determined to systematically decrease as the cross linking ion radius decreased. Consistent with the H-1-H-1 correlation spectra, regular structures were found for the gels cross linked by relatively large alkaline earth cations (Ba2+, Sr2+, or Ca2+), whereas the alginate chains cross-linked by bivalent transition metal ions (Zn") and trivalent metal cations (Al3+) exhibited significant irregularities. Notably, however, the observed disordering of the alginate chains was exclusively attributed to the M residues, whereas the structurally well-defined gels all contained guluronic acid (G) residues. Therefore, a key role of the units in M-rich blocks as mediators promoting the self assembly of alginate chains was experimentally confirmed. Finally, combining 2D Al-27 3Q/MAS NMR spectroscopy with density functional theory (DFT) calculations provided previously unreported insight into the structure of the Al3+ cross-linking centers. Notably, even with a low residual amount of water, these cross-linking units adopt exclusively 6-fold octahedral coordination and exhibit significant motion, which considerably reduces quadrupolar coupling constants. Thus, the experimental strategy presented in this study provides a new perspective on cross-linked alginate structure and dynamics for which high-quality diffraction data at the atomic resolution level are inherently unavailable.

Links

ED3.2.00/08.0144, research and development project

Name: CERIT Scientific Cloud

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BRUS, Jiří, Martina URBANOVA, Jiří CZERNEK, Miroslava PAVELKOVÁ, Kateřina KUBOVÁ, Jakub VYSLOUŽIL, Sabina ABBRENT, Rafal KONEFAL, Jiří HORSKY, David VETCHÝ, Jan VYSLOUŽIL and Pavel KULICH. Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy. Biomacromolecules. Washington: American Chemical Society, 2017, vol. 18, No 8, p. 2478-2488. ISSN 1525-7797. Available from: https://dx.doi.org/10.1021/acs.biomac.7b00627.

@article{1406167,
   author = {Brus, Jiří and Urbanova, Martina and Czernek, Jiří and Pavelková, Miroslava and Kubová, Kateřina and Vysloužil, Jakub and Abbrent, Sabina and Konefal, Rafal and Horsky, Jiří and Vetchý, David and Vysloužil, Jan and Kulich, Pavel},
   article_location = {Washington},
   article_number = {8},
   doi = {http://dx.doi.org/10.1021/acs.biomac.7b00627},
   keywords = {NUCLEAR-MAGNETIC-RESONANCE; EGG-BOX MODEL; SEGMENTAL DYNAMICS; DIVALENT-CATIONS; CALCIUM-ALGINATE; SODIUM ALGINATE; ACID; DELIVERY; COMPLEXATION; COMBINATION},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy},
   url = {https://pubs.acs.org/doi/abs/10.1021/acs.biomac.7b00627},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1406167
AU  - Brus, Jiří - Urbanova, Martina - Czernek, Jiří - Pavelková, Miroslava - Kubová, Kateřina - Vysloužil, Jakub - Abbrent, Sabina - Konefal, Rafal - Horsky, Jiří - Vetchý, David - Vysloužil, Jan - Kulich, Pavel
PY  - 2017
TI  - Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy
JF  - Biomacromolecules
VL  - 18
IS  - 8
SP  - 2478-2488
EP  - 2478-2488
PB  - American Chemical Society
SN  - 15257797
KW  - NUCLEAR-MAGNETIC-RESONANCE
KW  - EGG-BOX MODEL
KW  - SEGMENTAL DYNAMICS
KW  - DIVALENT-CATIONS
KW  - CALCIUM-ALGINATE
KW  - SODIUM ALGINATE
KW  - ACID
KW  - DELIVERY
KW  - COMPLEXATION
KW  - COMBINATION
UR  - https://pubs.acs.org/doi/abs/10.1021/acs.biomac.7b00627
L2  - https://pubs.acs.org/doi/abs/10.1021/acs.biomac.7b00627
N2  - Alginate gels are an outstanding biomaterial widely applicable in tissue engineering, medicine, and pharmacy agent delivery, respectively. This contribution provides new and comrehensive into the atomic-resolution structure for cell transplantation, wound healing and efficient bioactive agent delivery, respectively. This contribution provides new and comrehensive insight into the atomic-resolution structure and dynamics of polyvalent ion-cross-linked alginate gels in microbead formulations. By applying various advanced solid-state NMR (ssNMR) spectroscopy techniques, we verified the homogeneous distribution of the cross-linking ions in the alginate gels and the high degree of ion exchange. We also established that the two-component character of the alginate gels arises from the concentration fluctuations of residual water molecules that are preferentially localized along polymer chains containing abundant mannuronic acid (M) residues. These hydrated M-rich blocks tend to self-aggregate into subnanometer domains. The resulting coexistence of two types of alginate chains differing in segmental dynamics was revealed by H-1-C-13 dipolar profile analysis, which indicated that the average fluctuation angles of the stiff and mobile alginate segments were about 5-9 degrees or 30 degrees, respectively. Next, the C-13 CP/MAS NMR spectra indicated that the alginate polymer microstructure was strongly dependent on the type of cross-linking ion. The polymer chain regularity was determined to systematically decrease as the cross linking ion radius decreased. Consistent with the H-1-H-1 correlation spectra, regular structures were found for the gels cross linked by relatively large alkaline earth cations (Ba2+, Sr2+, or Ca2+), whereas the alginate chains cross-linked by bivalent transition metal ions (Zn") and trivalent metal cations (Al3+) exhibited significant irregularities. Notably, however, the observed disordering of the alginate chains was exclusively attributed to the M residues, whereas the structurally well-defined gels all contained guluronic acid (G) residues. Therefore, a key role of the units in M-rich blocks as mediators promoting the self assembly of alginate chains was experimentally confirmed. Finally, combining 2D Al-27 3Q/MAS NMR spectroscopy with density functional theory (DFT) calculations provided previously unreported insight into the structure of the Al3+ cross-linking centers. Notably, even with a low residual amount of water, these cross-linking units adopt exclusively 6-fold octahedral coordination and exhibit significant motion, which considerably reduces quadrupolar coupling constants. Thus, the experimental strategy presented in this study provides a new perspective on cross-linked alginate structure and dynamics for which high-quality diffraction data at the atomic resolution level are inherently unavailable.
ER  -

BRUS, Jiří, Martina URBANOVA, Jiří CZERNEK, Miroslava PAVELKOVÁ, Kateřina KUBOVÁ, Jakub VYSLOUŽIL, Sabina ABBRENT, Rafal KONEFAL, Jiří HORSKY, David VETCHÝ, Jan VYSLOUŽIL and Pavel KULICH. Structure and Dynamics of Alginate Gels Cross-Linked by Polyvalent Ions Probed via Solid State NMR Spectroscopy. \textit{Biomacromolecules}. Washington: American Chemical Society, 2017, vol.~18, No~8, p.~2478-2488. ISSN~1525-7797. Available from: https://dx.doi.org/10.1021/acs.biomac.7b00627.

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