CHLAPEK, Petr, Karel ZITTERBART, Leoš KŘEN, Lenka FILIPOVÁ, Jaroslav ŠTĚRBA a Renata VESELSKÁ. Uniformity under in vitro conditions: Changes in the phenotype of cancer cell lines derived from different medulloblastoma subgroups. PLOS ONE. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, roč. 12, č. 2, s. nestránkováno, 14 s. ISSN 1932-6203. doi:10.1371/journal.pone.0172552. 2017.
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Základní údaje
Originální název Uniformity under in vitro conditions: Changes in the phenotype of cancer cell lines derived from different medulloblastoma subgroups.
Autoři CHLAPEK, Petr (203 Česká republika, domácí), Karel ZITTERBART (203 Česká republika, domácí), Leoš KŘEN (203 Česká republika), Lenka FILIPOVÁ (203 Česká republika, domácí), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Renata VESELSKÁ (203 Česká republika, garant, domácí).
Vydání PLOS ONE, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2017, 1932-6203.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10601 Cell biology
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 2.766
Kód RIV RIV/00216224:14310/17:00099806
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.1371/journal.pone.0172552
UT WoS 000394682400055
Klíčová slova anglicky medulloblastoma; molecular subgroups; primary tumors; cell lines; beta-catenin; filamin A; GAB1; YAP1; immunodetection; protein markers
Štítky NZ, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Nicole Zrilić, učo 240776. Změněno: 12. 4. 2018 17:41.
Anotace
Medulloblastoma comprises four main subgroups (WNT, SHH, Group 3 and Group 4) originally defined by transcriptional profiling. In primary medulloblastoma tissues, these groups are thought to be distinguishable using the immunohistochemical detection of beta-catenin, filamin A, GAB1 and YAP1 protein markers. To investigate the utility of these markers for in vitro studies using medulloblastoma cell lines, immunoblotting and indirect immunofluorescence were employed for the detection of beta-catenin, filamin A, GAB1 and YAP1 in both DAOY and D283 Med reference cell lines and the panel of six medulloblastoma cell lines derived in our laboratory from the primary tumor tissues of known molecular subgroups. Immunohistochemical detection of these markers was performed on formalin-fixed paraffin-embedded tissue of the matching primary tumors. The results revealed substantial divergences between the primary tumor tissues and matching cell lines in the immunoreactivity pattern of medulloblastoma-subgroup-specific protein markers. Regardless of the molecular subgroup of the primary tumor, all six patient-derived medulloblastoma cell lines exhibited a uniform phenotype: immunofluorescence showed the nuclear localization of YAP1, accompanied by strong cytoplasmic positivity for beta-catenin and filamin A, as well as weak positivity for GAB1. The same immunoreactivity pattern was also found in both DAOY and D283 Med reference medulloblastoma cell lines. Therefore, we can conclude that various medulloblastoma cell lines tend to exhibit the same characteristics of protein marker expression under standard in vitro conditions. Such a finding emphasizes the importance of the analyses of primary tumors in clinically oriented medulloblastoma research and the urgent need to develop in vitro models of improved clinical relevance, such as 3D cultures and organotypic slice cultures.
VytisknoutZobrazeno: 28. 3. 2024 21:50