Maternal white blood cell count cannot identify the presence of
microbial invasion of the amniotic cavity or intra-amniotic
inflammation in women with preterm prelabor rupture of
membranes

J 2017

Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes

MUSILOVA, I., L. PLISKOVA, Romana GERYCHOVÁ, Petr JANKŮ, O. SIMETKA et. al.

Basic information

Original name

Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes

Authors

MUSILOVA, I. (203 Czech Republic), L. PLISKOVA (203 Czech Republic), Romana GERYCHOVÁ (203 Czech Republic, belonging to the institution), Petr JANKŮ (203 Czech Republic, belonging to the institution), O. SIMETKA (203 Czech Republic), P. MATLAK (203 Czech Republic), B. JACOBSSON (752 Sweden) and M. KACEROVSKY (203 Czech Republic)

Edition

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14110/17:00099904

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0189394

UT WoS

000417698200039

Keywords in English

maternal white blood cell

Abstract

V originále

The main aim of this study was to determine the relationship between the maternal white blood cell (WBC) count at the time of hospital admission in pregnancies complicated by pre-term prelabor rupture of membranes (PPROM) and the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). The second aim was to test WBC diagnostic indices with respect to the presence of MIAC and/or IAI. Four hundred and seventy-nine women with singleton pregnancies complicated by PPROM, between February 2012 and June 2017, were included in this study. Maternal blood and amniotic fluid samples were collected at the time of admission. Maternal WBC count was assessed. Amniotic fluid interleukin-6 (IL-6) concentration was measured using a point-of-care test, and IAI was characterized by an IL-6 concentration of >= 745 pg/mL. MIAC was diagnosed based on a positive polymerase chain reaction result for the Ureaplasma species, Mycoplasma hominis, and/or Chlamydia trachomatis and/or for the 16S rRNA gene. Women with MIAC or IAI had higher WBC counts than those without (with MIAC: median, 12.8 x 10(9) /L vs. without MIAC: median, 11.9 x 10(9) /L; p = 0.0006; with IAI: median, 13.7 x 10(9) /L vs. without IAI: median, 11.9 x 10(9)/L; p < 0.0001). When the women were divided into four sub-groups based on the presence of MIAC and/or IAI, the women with both MIAC and IAI had a higher WBC count than those with either IAI or MIAC alone, and those without MIAC and IAI [both MIAC and IAI: median, 14.0 x 10(9) /L; IAI alone: 12.1 x 10(9) /L (p = 0.03); MIAC alone: 12.1 x 10(9)/L (p = 0.0001); and without MIAC and IAI: median, 11.8 x 10(9)/L (p < 0.0001)]. No differences in the WBC counts were found among the women with IAI alone, MIAC alone, and without MIAC and IAI. The women with both MIAC and IAI had a higher maternal WBC count at the time of hospital admission than the remaining women with PPROM. The maternal WBC count at the time of admission showed poor diagnostic indices for the identification of the presence of both MIAC and IAI. Maternal WBC count at the time of admission cannot serve as a non-invasive screening tool for identifying these complications in women with PPROM.

Citovat

MUSILOVA, I., L. PLISKOVA, Romana GERYCHOVÁ, Petr JANKŮ, O. SIMETKA, P. MATLAK, B. JACOBSSON and M. KACEROVSKY. Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes. Plos one. San Francisco: Public Library of Science, 2017, vol. 12, No 12, p. 1-17. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0189394.

@article{1406914,
   author = {Musilova, I. and Pliskova, L. and Gerychová, Romana and Janků, Petr and Simetka, O. and Matlak, P. and Jacobsson, B. and Kacerovsky, M.},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pone.0189394},
   keywords = {maternal white blood cell},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1406914
AU  - Musilova, I. - Pliskova, L. - Gerychová, Romana - Janků, Petr - Simetka, O. - Matlak, P. - Jacobsson, B. - Kacerovsky, M.
PY  - 2017
TI  - Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes
JF  - Plos one
VL  - 12
IS  - 12
SP  - 1-17
EP  - 1-17
PB  - Public Library of Science
SN  - 19326203
KW  - maternal white blood cell
N2  - The main aim of this study was to determine the relationship between the maternal white blood cell (WBC) count at the time of hospital admission in pregnancies complicated by pre-term prelabor rupture of membranes (PPROM) and the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). The second aim was to test WBC diagnostic indices with respect to the presence of MIAC and/or IAI. Four hundred and seventy-nine women with singleton pregnancies complicated by PPROM, between February 2012 and June 2017, were included in this study. Maternal blood and amniotic fluid samples were collected at the time of admission. Maternal WBC count was assessed. Amniotic fluid interleukin-6 (IL-6) concentration was measured using a point-of-care test, and IAI was characterized by an IL-6 concentration of >= 745 pg/mL. MIAC was diagnosed based on a positive polymerase chain reaction result for the Ureaplasma species, Mycoplasma hominis, and/or Chlamydia trachomatis and/or for the 16S rRNA gene. Women with MIAC or IAI had higher WBC counts than those without (with MIAC: median, 12.8 x 10(9) /L vs. without MIAC: median, 11.9 x 10(9) /L; p = 0.0006; with IAI: median, 13.7 x 10(9) /L vs. without IAI: median, 11.9 x 10(9)/L; p < 0.0001). When the women were divided into four sub-groups based on the presence of MIAC and/or IAI, the women with both MIAC and IAI had a higher WBC count than those with either IAI or MIAC alone, and those without MIAC and IAI [both MIAC and IAI: median, 14.0 x 10(9) /L; IAI alone: 12.1 x 10(9) /L (p = 0.03); MIAC alone: 12.1 x 10(9)/L (p = 0.0001); and without MIAC and IAI: median, 11.8 x 10(9)/L (p < 0.0001)]. No differences in the WBC counts were found among the women with IAI alone, MIAC alone, and without MIAC and IAI. The women with both MIAC and IAI had a higher maternal WBC count at the time of hospital admission than the remaining women with PPROM. The maternal WBC count at the time of admission showed poor diagnostic indices for the identification of the presence of both MIAC and IAI. Maternal WBC count at the time of admission cannot serve as a non-invasive screening tool for identifying these complications in women with PPROM.
ER  -

MUSILOVA, I., L. PLISKOVA, Romana GERYCHOVÁ, Petr JANKŮ, O. SIMETKA, P. MATLAK, B. JACOBSSON and M. KACEROVSKY. Maternal white blood cell count cannot identify the presence of microbial invasion of the amniotic cavity or intra-amniotic inflammation in women with preterm prelabor rupture of membranes. \textit{Plos one}. San Francisco: Public Library of Science, 2017, vol.~12, No~12, p.~1-17. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0189394.

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