Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich
Nanozyme-Linked Immunosorbent Assay

J 2018

Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay

FARKA, Zdeněk, Veronika ČUNDERLOVÁ, Veronika HORÁČKOVÁ, Matěj PASTUCHA, Zuzana MIKUŠOVÁ et. al.

Basic information

Original name

Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution), Veronika ČUNDERLOVÁ (203 Czech Republic, belonging to the institution), Veronika HORÁČKOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, DC, American Chemical Society, 2018, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.350

RIV identification code

RIV/00216224:14740/18:00100815

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acs.analchem.7b04883

UT WoS

000424730600119

Keywords in English

Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin

Abstract

V originále

Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3-based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensely blue product. Here, we introduce the method for conjugation of PBNPs with antibodies and their application in nanozyme-linked immunosorbent assay (NLISA). Sandwich NLISA for detection of human serum albumin in urine was developed with limit of detection (LOD) of 1.2 ng·mL–1 and working range up to 1 ug·mL-1. Furthermore, the microbial contamination of Salmonella Typhimurium in powdered milk was detected with LOD of 6 × 10^3 colony-forming units (cfu)·mL-1 and working range up to 10^6 cfu·mL-1. In both cases, a critical comparison with the same immunoassay but using native peroxidase as label was realized. The achieved results confirmed the suitability of PBNPs for universal and robust replacement of enzyme labels.

Links

GBP206/12/G014, research and development project

Name: Centrum pokročilých bioanalytických technologií

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

FARKA, Zdeněk, Veronika ČUNDERLOVÁ, Veronika HORÁČKOVÁ, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK and Petr SKLÁDAL. Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay. Analytical Chemistry. Washington, DC: American Chemical Society, 2018, vol. 90, No 3, p. 2348-2354. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.7b04883.

@article{1407052,
   author = {Farka, Zdeněk and Čunderlová, Veronika and Horáčková, Veronika and Pastucha, Matěj and Mikušová, Zuzana and Hlaváček, Antonín and Skládal, Petr},
   article_location = {Washington, DC},
   article_number = {3},
   doi = {http://dx.doi.org/10.1021/acs.analchem.7b04883},
   keywords = {Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.7b04883},
   volume = {90},
   year = {2018}
}

TY  - JOUR
ID  - 1407052
AU  - Farka, Zdeněk - Čunderlová, Veronika - Horáčková, Veronika - Pastucha, Matěj - Mikušová, Zuzana - Hlaváček, Antonín - Skládal, Petr
PY  - 2018
TI  - Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay
JF  - Analytical Chemistry
VL  - 90
IS  - 3
SP  - 2348-2354
EP  - 2348-2354
PB  - American Chemical Society
SN  - 00032700
KW  - Prussian blue nanoparticle
KW  - Enzyme biomimic
KW  - Bioconjugation
KW  - Sandwich immunoassay
KW  - Salmonella
KW  - Human serum albumin
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.7b04883
N2  - Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3-based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensely blue product. Here, we introduce the method for conjugation of PBNPs with antibodies and their application in nanozyme-linked immunosorbent assay (NLISA). Sandwich NLISA for detection of human serum albumin in urine was developed with limit of detection (LOD) of 1.2 ng·mL–1 and working range up to 1 ug·mL-1. Furthermore, the microbial contamination of Salmonella Typhimurium in powdered milk was detected with LOD of 6 × 10^3 colony-forming units (cfu)·mL-1 and working range up to 10^6 cfu·mL-1. In both cases, a critical comparison with the same immunoassay but using native peroxidase as label was realized. The achieved results confirmed the suitability of PBNPs for universal and robust replacement of enzyme labels.
ER  -

FARKA, Zdeněk, Veronika ČUNDERLOVÁ, Veronika HORÁČKOVÁ, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK and Petr SKLÁDAL. Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2018, vol.~90, No~3, p.~2348-2354. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.7b04883.

Detailed Information on Publication Record