FARKA, Zdeněk, Veronika ČUNDERLOVÁ, Veronika HORÁČKOVÁ, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK and Petr SKLÁDAL. Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay. Analytical Chemistry. Washington, DC: American Chemical Society, 2018, vol. 90, No 3, p. 2348-2354. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.7b04883.
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Basic information
Original name Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay
Authors FARKA, Zdeněk (203 Czech Republic, belonging to the institution), Veronika ČUNDERLOVÁ (203 Czech Republic, belonging to the institution), Veronika HORÁČKOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
Edition Analytical Chemistry, Washington, DC, American Chemical Society, 2018, 0003-2700.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 6.350
RIV identification code RIV/00216224:14740/18:00100815
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1021/acs.analchem.7b04883
UT WoS 000424730600119
Keywords in English Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin
Tags CF CRYO, CF NANO, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 19/3/2019 09:07.
Abstract
Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3-based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensely blue product. Here, we introduce the method for conjugation of PBNPs with antibodies and their application in nanozyme-linked immunosorbent assay (NLISA). Sandwich NLISA for detection of human serum albumin in urine was developed with limit of detection (LOD) of 1.2 ng·mL–1 and working range up to 1 ug·mL-1. Furthermore, the microbial contamination of Salmonella Typhimurium in powdered milk was detected with LOD of 6 × 10^3 colony-forming units (cfu)·mL-1 and working range up to 10^6 cfu·mL-1. In both cases, a critical comparison with the same immunoassay but using native peroxidase as label was realized. The achieved results confirmed the suitability of PBNPs for universal and robust replacement of enzyme labels.
Links
GBP206/12/G014, research and development projectName: Centrum pokročilých bioanalytických technologií
LM2015043, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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