Kinetic properties of growth of intestinal sulphate-reducing
bacteria isolated from healthy mice and mice with ulcerative
colitis

J 2017

Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis

KUSHKEVYCH, Ivan, Monika VÍTĚZOVÁ, Pavla FEDROVÁ, Zora VOCHYANOVÁ, Lenka PARÁKOVÁ et. al.

Basic information

Original name

Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis

Authors

KUSHKEVYCH, Ivan (804 Ukraine, belonging to the institution), Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution), Pavla FEDROVÁ (203 Czech Republic, belonging to the institution), Zora VOCHYANOVÁ (203 Czech Republic), Lenka PARÁKOVÁ (203 Czech Republic) and Jan HOŠEK (203 Czech Republic)

Edition

Acta Veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2017, 0001-7213

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.422

RIV identification code

RIV/00216224:14310/17:00099928

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.2754/avb201786040405

UT WoS

000427451500013

Keywords in English

Intestinal microbiota; growth rate; hydrogen sulphide; bowel diseases

Abstract

V originále

Inflammatory bowel disease including ulcerative colitis are complex multifactorial diseases of unknown aetiology. Sulphate-reducing bacteria are often associated with the occurrence of the disease. The physiological properties of intestinal sulphate-reducing bacteria including kinetic characteristic of their growth have never been reported. The aim of this research was to evaluate the presence of sulphate-reducing bacteria isolated from the intestines of mice, study their growth, calculate and compare the kinetic growth properties on the model of dextran sulphate sodium induced ulcerative colitis in the mice. The number of viable intestinal sulphate-reducing bacteria from the bowel lumen of mice with ulcerative colitis was higher (P > 0.05) by 22% at 12 h of cultivation compared with cultures of sulphate-reducing bacteria from the bowel lumen of healthy mice. The sulphate-reducing bacteria from mice with colitis also had a slightly higher generation time (14.29 h) and exponential growth phase (22.24 h) compared with cultures from healthy mice. The time of lag-phase was 2 × shorter (P > 0.01) in the cultures of sulphate-reducing bacteria from mice with ulcerative colitis. The described research is new and important for the prediction of the sulphate-reducing bacteria number in the gut and their rate of dissimilatory sulphate reduction. The kinetic characteristic of their growth is important for further clarification of the mechanisms of sulphate reduction and accumulation of hydrogen sulphide, which is toxic for epithelial cells of the intestine and can cause bowel diseases both in humans and animals, in particular ulcerative colitis.

Links

MUNI/A/0906/2016, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 1 (Acronym: SVMIK)

Investor: Masaryk University, Category A

Citovat

KUSHKEVYCH, Ivan, Monika VÍTĚZOVÁ, Pavla FEDROVÁ, Zora VOCHYANOVÁ, Lenka PARÁKOVÁ and Jan HOŠEK. Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis. Acta Veterinaria Brno. Brno: Veterinární a farmaceutická univerzita Brno, 2017, vol. 86, No 4, p. 405-411. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201786040405.

@article{1407191,
   author = {Kushkevych, Ivan and Vítězová, Monika and Fedrová, Pavla and Vochyanová, Zora and Paráková, Lenka and Hošek, Jan},
   article_location = {Brno},
   article_number = {4},
   doi = {http://dx.doi.org/10.2754/avb201786040405},
   keywords = {Intestinal microbiota; growth rate; hydrogen sulphide; bowel diseases},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria Brno},
   title = {Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis},
   url = {https://actavet.vfu.cz/86/4/0405/},
   volume = {86},
   year = {2017}
}

TY  - JOUR
ID  - 1407191
AU  - Kushkevych, Ivan - Vítězová, Monika - Fedrová, Pavla - Vochyanová, Zora - Paráková, Lenka - Hošek, Jan
PY  - 2017
TI  - Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis
JF  - Acta Veterinaria Brno
VL  - 86
IS  - 4
SP  - 405-411
EP  - 405-411
PB  - Veterinární a farmaceutická univerzita Brno
SN  - 00017213
KW  - Intestinal microbiota
KW  - growth rate
KW  - hydrogen sulphide
KW  - bowel diseases
UR  - https://actavet.vfu.cz/86/4/0405/
L2  - https://actavet.vfu.cz/86/4/0405/
N2  - Inflammatory bowel disease including ulcerative colitis are complex multifactorial diseases of unknown aetiology. Sulphate-reducing bacteria are often associated with the occurrence of the disease. The physiological properties of intestinal sulphate-reducing bacteria including kinetic characteristic of their growth have never been reported. The aim of this research was to evaluate the presence of sulphate-reducing bacteria isolated from the intestines of mice, study their growth, calculate and compare the kinetic growth properties on the model of dextran sulphate sodium induced ulcerative colitis in the mice. The number of viable intestinal sulphate-reducing bacteria from the bowel lumen of mice with ulcerative colitis was higher (P > 0.05) by 22% at 12 h of cultivation compared with cultures of sulphate-reducing bacteria from the bowel lumen of healthy mice. The sulphate-reducing bacteria from mice with colitis also had a slightly higher generation time (14.29 h) and exponential growth phase (22.24 h) compared with cultures from healthy mice. The time of lag-phase was 2 × shorter (P > 0.01) in the cultures of sulphate-reducing bacteria from mice with ulcerative colitis. The described research is new and important for the prediction of the sulphate-reducing bacteria number in the gut and their rate of dissimilatory sulphate reduction. The kinetic characteristic of their growth is important for further clarification of the mechanisms of sulphate reduction and accumulation of hydrogen sulphide, which is toxic for epithelial cells of the intestine and can cause bowel diseases both in humans and animals, in particular ulcerative colitis.
ER  -

KUSHKEVYCH, Ivan, Monika VÍTĚZOVÁ, Pavla FEDROVÁ, Zora VOCHYANOVÁ, Lenka PARÁKOVÁ and Jan HOŠEK. Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis. \textit{Acta Veterinaria Brno}. Brno: Veterinární a farmaceutická univerzita Brno, 2017, vol.~86, No~4, p.~405-411. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb201786040405.

Detailed Information on Publication Record