J 2017

The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand

SMRČKA, Filip, Přemysl LUBAL and Michal ŠÍDLO

Basic information

Original name

The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand

Authors

SMRČKA, Filip (203 Czech Republic, belonging to the institution), Přemysl LUBAL (203 Czech Republic, guarantor, belonging to the institution) and Michal ŠÍDLO (203 Czech Republic, belonging to the institution)

Edition

Monatshefte fur Chemie, Wien, Springer Wien, 2017, 0026-9247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.285

RIV identification code

RIV/00216224:14310/17:00095481

Organization unit

Faculty of Science

DOI

UT WoS

000413626000008

Keywords in English

Luminescent Eu(III) complex; Biosensor; Carbonate; Urease enzyme; Urea determination

Tags

Tags

International impact, Reviewed
Změněno: 12/4/2018 15:19, Ing. Nicole Zrilić

Abstract

V originále

The Eu(III) ternary complex with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (H(3)DO3A) and picolinate "antenna" ligand ([Eu(DO3A)(L)](-)) reacts with carbonate anions to form the more stable non-fluorescent [Eu(DO3A)(CO3)](2-) complex. This substitution reaction was employed for indirect determination of carbonate formed in course of urea hydrolysis catalyzed by the urease enzyme. This new analytical procedure was proposed for the determination of both analytes (urea, urease) and this sensitive luminescence-based biosensor can be used in both kinetic time-dependent and equilibrium end-point modes. This method, with analytical figures of merit (limit of detection 0.05 mM, dynamic range 0.05-5.00 mM), does not show any systematic error and therefore can be utilized for urea analysis in biological samples (e.g., urine). It was also demonstrated that some metal ions (e.g., Zn(II), Pb(II), Cd(II), Ag(I), etc.) inhibit the enzymatic hydrolysis of urea and this effect can be utilized for their determination; therefore, it is recommended to add EDTA as masking agent prior to enzymatic urea analysis.

Links

GA13-08336S, research and development project
Name: Hybridní materiály založené na makrocyklických ligandech pro využití v medicíně
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1237/2016, interní kód MU
Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017
Investor: Masaryk University, Category A
692034, interní kód MU
Name: TWINFUSYON - Twinning for Improving Capacity of Research in Multifunctional Nanosystems for Optronic Biosensing (Acronym: TWINFUSYON)
Investor: European Union, Spreading excellence and widening participation
692298, interní kód MU
Name: MEDGENET - Medical genomics and epigenomics network (Acronym: MEDGENET)
Investor: European Union, Spreading excellence and widening participation