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@article{1407226,
author = {Smrčka, Filip and Lubal, Přemysl and Šídlo, Michal},
article_location = {Wien},
article_number = {11},
doi = {http://dx.doi.org/10.1007/s00706-017-2043-4},
keywords = {Luminescent Eu(III) complex; Biosensor; Carbonate; Urease enzyme; Urea determination},
language = {eng},
issn = {0026-9247},
journal = {Monatshefte fur Chemie},
title = {The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand},
url = {https://link.springer.com/article/10.1007%2Fs00706-017-2043-4},
volume = {148},
year = {2017}
}
TY - JOUR
ID - 1407226
AU - Smrčka, Filip - Lubal, Přemysl - Šídlo, Michal
PY - 2017
TI - The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand
JF - Monatshefte fur Chemie
VL - 148
IS - 11
SP - 1945-1952
EP - 1945-1952
PB - Springer Wien
SN - 00269247
KW - Luminescent Eu(III) complex
KW - Biosensor
KW - Carbonate
KW - Urease enzyme
KW - Urea determination
UR - https://link.springer.com/article/10.1007%2Fs00706-017-2043-4
L2 - https://link.springer.com/article/10.1007%2Fs00706-017-2043-4
N2 - The Eu(III) ternary complex with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (H(3)DO3A) and picolinate "antenna" ligand ([Eu(DO3A)(L)](-)) reacts with carbonate anions to form the more stable non-fluorescent [Eu(DO3A)(CO3)](2-) complex. This substitution reaction was employed for indirect determination of carbonate formed in course of urea hydrolysis catalyzed by the urease enzyme. This new analytical procedure was proposed for the determination of both analytes (urea, urease) and this sensitive luminescence-based biosensor can be used in both kinetic time-dependent and equilibrium end-point modes. This method, with analytical figures of merit (limit of detection 0.05 mM, dynamic range 0.05-5.00 mM), does not show any systematic error and therefore can be utilized for urea analysis in biological samples (e.g., urine). It was also demonstrated that some metal ions (e.g., Zn(II), Pb(II), Cd(II), Ag(I), etc.) inhibit the enzymatic hydrolysis of urea and this effect can be utilized for their determination; therefore, it is recommended to add EDTA as masking agent prior to enzymatic urea analysis.
ER -
SMRČKA, Filip, Přemysl LUBAL and Michal ŠÍDLO. The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand. \textit{Monatshefte fur Chemie}. Wien: Springer Wien, 2017, vol.~148, No~11, p.~1945-1952. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-017-2043-4.
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