Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

SUN, Xiaolong, Maria L. ODYNIEC, Adam C. SEDGWICK, Karel LACINA, Suying XU, Taotao QIANG, Steven D. BULL, Frank MARKEN and Tony D. JAMES. Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide. Organic Chemistry Frontiers. 2017, vol. 4, No 6, p. 1058-1062. ISSN 2052-4129. Available from: https://dx.doi.org/10.1039/c6qo00448b.

Basic information

• Original name: Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide
• Authors: SUN, Xiaolong (156 China), Maria L. ODYNIEC (826 United Kingdom of Great Britain and Northern Ireland), Adam C. SEDGWICK (826 United Kingdom of Great Britain and Northern Ireland), Karel LACINA (203 Czech Republic, guarantor, belonging to the institution), Suying XU (156 China), Taotao QIANG (156 China), Steven D. BULL (826 United Kingdom of Great Britain and Northern Ireland), Frank MARKEN (826 United Kingdom of Great Britain and Northern Ireland) and Tony D. JAMES (826 United Kingdom of Great Britain and Northern Ireland).
• Edition: Organic Chemistry Frontiers, 2017, 2052-4129.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10401 Organic chemistry
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.455
• RIV identification code: RIV/00216224:14740/17:00099973
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1039/c6qo00448b
• UT WoS: 000405862600021
• Keywords in English: FLUORESCENT-PROBES; LIVING CELLS; BORONIC ACID; IN-VIVO; INFLAMMATION; REPORTER; MODEL
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2 via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR