Antibody-mediated modulation of cytokinins in tobacco:
organ-specific changes in cytokinin homeostasis

J 2018

Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis

GELOVÁ, Zuzana, Petra TEN HOOPEN, Ondřej NOVÁK, Václav MOTYKA, Markéta PERNISOVÁ et. al.

Basic information

Original name

Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis

Authors

GELOVÁ, Zuzana (203 Czech Republic, belonging to the institution), Petra TEN HOOPEN (276 Germany), Ondřej NOVÁK (203 Czech Republic), Václav MOTYKA (203 Czech Republic), Markéta PERNISOVÁ (203 Czech Republic, belonging to the institution), Siarhei DABRAVOLSKI (112 Belarus, belonging to the institution), Vojtěch DIDI (203 Czech Republic, belonging to the institution), Isolde TILLACK (276 Germany), Jana OKLEŠŤKOVÁ (203 Czech Republic), Miroslav STRNAD (203 Czech Republic), Bettina HAUSE (276 Germany), Danka HARUŠTIAKOVÁ (703 Slovakia, belonging to the institution), Udo CONRAD (276 Germany), Lubomír JANDA (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Experimental Botany, Clarendon Press, 2018, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.360

RIV identification code

RIV/00216224:14740/18:00100822

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/jxb/erx426

UT WoS

000423706500009

Keywords in English

antibody-mediated modulation; biosynthesis; CKX; cytokinin; homeostasis; organ specificity; tobacco.

Abstract

V originále

Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation.

Links

GA13-25280S, research and development project

Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Czech Science Foundation

GP14-30004P, research and development project

Name: Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.

Investor: Czech Science Foundation

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

GELOVÁ, Zuzana, Petra TEN HOOPEN, Ondřej NOVÁK, Václav MOTYKA, Markéta PERNISOVÁ, Siarhei DABRAVOLSKI, Vojtěch DIDI, Isolde TILLACK, Jana OKLEŠŤKOVÁ, Miroslav STRNAD, Bettina HAUSE, Danka HARUŠTIAKOVÁ, Udo CONRAD, Lubomír JANDA and Jan HEJÁTKO. Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. Journal of Experimental Botany. Clarendon Press, 2018, vol. 69, No 3, p. 441-454. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erx426.

@article{1407869,
   author = {Gelová, Zuzana and ten Hoopen, Petra and Novák, Ondřej and Motyka, Václav and Pernisová, Markéta and Dabravolski, Siarhei and Didi, Vojtěch and Tillack, Isolde and Oklešťková, Jana and Strnad, Miroslav and Hause, Bettina and Haruštiaková, Danka and Conrad, Udo and Janda, Lubomír and Hejátko, Jan},
   article_number = {3},
   doi = {http://dx.doi.org/10.1093/jxb/erx426},
   keywords = {antibody-mediated modulation; biosynthesis; CKX; cytokinin; homeostasis; organ specificity; tobacco.},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis},
   volume = {69},
   year = {2018}
}

TY  - JOUR
ID  - 1407869
AU  - Gelová, Zuzana - ten Hoopen, Petra - Novák, Ondřej - Motyka, Václav - Pernisová, Markéta - Dabravolski, Siarhei - Didi, Vojtěch - Tillack, Isolde - Oklešťková, Jana - Strnad, Miroslav - Hause, Bettina - Haruštiaková, Danka - Conrad, Udo - Janda, Lubomír - Hejátko, Jan
PY  - 2018
TI  - Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis
JF  - Journal of Experimental Botany
VL  - 69
IS  - 3
SP  - 441-454
EP  - 441-454
PB  - Clarendon Press
SN  - 00220957
KW  - antibody-mediated modulation
KW  - biosynthesis
KW  - CKX
KW  - cytokinin
KW  - homeostasis
KW  - organ specificity
KW  - tobacco.
N2  - Cytokinins comprise a group of phytohormones with an organ-specific mode of action. Although the mechanisms controlling the complex networks of cytokinin metabolism are partially known, the role of individual cytokinin types in the maintenance of cytokinin homeostasis remains unclear. Utilizing the overproduction of single-chain Fv antibodies selected for their ability to bind trans-zeatin riboside and targeted to the endoplasmic reticulum, we post-synthetically modulated cytokinin ribosides, the proposed transport forms of cytokinins. We observed asymmetric activity of cytokinin biosynthetic genes and cytokinin distribution in wild-type tobacco seedlings with higher cytokinin abundance in the root than in the shoot. Antibody-mediated modulation of cytokinin ribosides further enhanced the relative cytokinin abundance in the roots and induced cytokinin-related phenotypes in an organ-specific manner. The activity of cytokinin oxidase/dehydrogenase in the roots was strongly up-regulated in response to antibody-mediated formation of the cytokinin pool in the endoplasmic reticulum. However, we only detected a slight decrease in the root cytokinin levels. In contrast, a significant decrease of cytokinins occurred in the shoot. We suggest the roots as the main site of cytokinin biosynthesis in tobacco seedlings. Conversely, cytokinin levels in the shoot seem to depend largely on long-range transport of cytokinin ribosides from the root and their subsequent metabolic activation.
ER  -

GELOVÁ, Zuzana, Petra TEN HOOPEN, Ondřej NOVÁK, Václav MOTYKA, Markéta PERNISOVÁ, Siarhei DABRAVOLSKI, Vojtěch DIDI, Isolde TILLACK, Jana OKLEŠŤKOVÁ, Miroslav STRNAD, Bettina HAUSE, Danka HARUŠTIAKOVÁ, Udo CONRAD, Lubomír JANDA and Jan HEJÁTKO. Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. \textit{Journal of Experimental Botany}. Clarendon Press, 2018, vol.~69, No~3, p.~441-454. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erx426.

Detailed Information on Publication Record