J 2017

Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)

KILLER, Jiří, Radko PECHAR, Pavel ŠVEC, Hana SALMONOVÁ, Roman ŠVEJSTIL et. al.

Basic information

Original name

Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)

Authors

KILLER, Jiří (203 Czech Republic), Radko PECHAR (203 Czech Republic), Pavel ŠVEC (203 Czech Republic, guarantor, belonging to the institution), Hana SALMONOVÁ (203 Czech Republic), Roman ŠVEJSTIL (203 Czech Republic), Martina GEIGEROVÁ (203 Czech Republic), Vojtěch RADA (203 Czech Republic), Eva VLKOVÁ (203 Czech Republic) and Chahrazed MEKADIM (12 Algeria)

Edition

International Journal of Systematic and Evolutionary Microbiology, London, Microbiology Society, 2017, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.932

RIV identification code

RIV/00216224:14310/17:00099979

Organization unit

Faculty of Science

DOI

UT WoS

000409533100064

Keywords in English

Lactobacillus sp. nov.; oral cavity; guinea pig; MLSA

Tags

Změněno: 6/4/2018 10:58, Ing. Nicole Zrilić

Abstract

V originále

A Gram-stain-positive, facultatively anaerobic, and catalase-and oxidase-negative bacterial strain designated MOZM2(T), having 98.4% 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016(T), was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42% degree of genetic similarity between the novel strain and L. reuteri DSM 20016(T). Strain MOZM2(T) degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2(T) and L. reuteri DSM 20016(T) in their proportions of C-8:0, C-14:1, C-17:0, C-18:2 omega 6t and C-20:0 fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G +C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name Lactobacillus caviae sp. nov. is proposed, of which the type strain is MOZM2(T) (= CCM 8609(T) = DSM 100239(T) = LMG 28780(T)).