New probabilistic risk assessment of ethylhexyl
methoxycinnamate: Comparing the genotoxic effects of trans- and
cis-EHMC

J 2017

New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC

SHARMA, Anežka, Katarína BÁNYIOVÁ, Jaromír LITERÁK and Pavel ČUPR

Basic information

Original name

New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC

Authors

SHARMA, Anežka (203 Czech Republic, belonging to the institution), Katarína BÁNYIOVÁ (703 Slovakia, belonging to the institution), Jaromír LITERÁK (203 Czech Republic, belonging to the institution) and Pavel ČUPR (203 Czech Republic, guarantor, belonging to the institution)

Edition

Environmental toxicology, Hoboken, USA, WILEY-BLACKWELL, 2017, 1520-4081

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10503 Water resources

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.491

RIV identification code

RIV/00216224:14310/17:00095508

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/tox.22260

UT WoS

000393799500019

Keywords in English

ethylhexyl methoxycinnamate; trans/cis isomerisation; genotoxicity; probabilistic risk assessment; QIVIVE

Abstract

V originále

Ethylhexyl methoxycinnamate (EHMC) is a widely used UV filter present in a large number of personal care products (PCPs). Under normal conditions, EHMC occurs in a mixture of two isomers: trans-EHMC and cis-EHMC in a ratio of 99:1. When exposed to sunlight, the trans isomer is transformed to the less stable cis isomer and the efficiency of the UV filter is reduced. To date, the toxicological effects of the cis-EHMC isomer remain largely unknown. We developed a completely new method for preparing cis-EHMC. An EHMC technical mixture was irradiated using a UV lamp and 98% pure cis-EHMC was isolated from the irradiated solution using column chromatography. The genotoxic effects of the isolated cis-EHMC isomer and the nonirradiated trans-EHMC were subsequently measured using two bioassays (SOS chromotest and UmuC test). In the case of trans-EHMC, significant genotoxicity was observed using both bioassays at the highest concentrations (0.5 - 4 mgmL(-1)). In the case of cis-EHMC, significant genotoxicity was only detected using the UmuC test at concentrations of 0.25 - 1 mgmL(-1). Based on these results, the NOEC was calculated for both cis- and trans-EHMC, 0.038 and 0.064 mgmL(-1), respectively. Risk assessment of dermal, oral and inhalation exposure to PCPs containing EHMC was carried out for a female population using probabilistic simulation and by using Quantitative in vitro to in vivo extrapolation (QIVIVE). The risk of cis-EHMC was found to be approximate to 1.7 times greater than trans-EHMC. In the case of cis-EHMC, a hazard index of 1 was exceeded in the 92nd percentile. Based on the observed differences between the isomers, EHMC application in PCPs requires detailed reassessment. Further exploration of the toxicological effects and properties of cis-EHMC is needed in order to correctly predict risks posed to humans and the environment.

Links

GA14-27941S, research and development project

Name: Kinetika perkutánní penetrace optických izomerů filtrů ultrafialového záření, bromovaných retardérů hoření a polárních pesticidů při dermální expozici

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SHARMA, Anežka, Katarína BÁNYIOVÁ, Jaromír LITERÁK and Pavel ČUPR. New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC. Environmental toxicology. Hoboken, USA: WILEY-BLACKWELL, 2017, vol. 32, No 2, p. 569-580. ISSN 1520-4081. Available from: https://dx.doi.org/10.1002/tox.22260.

@article{1408672,
   author = {Sharma, Anežka and Bányiová, Katarína and Literák, Jaromír and Čupr, Pavel},
   article_location = {Hoboken, USA},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/tox.22260},
   keywords = {ethylhexyl methoxycinnamate; trans/cis isomerisation; genotoxicity; probabilistic risk assessment; QIVIVE},
   language = {eng},
   issn = {1520-4081},
   journal = {Environmental toxicology},
   title = {New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/tox.22260/abstract},
   volume = {32},
   year = {2017}
}

TY  - JOUR
ID  - 1408672
AU  - Sharma, Anežka - Bányiová, Katarína - Literák, Jaromír - Čupr, Pavel
PY  - 2017
TI  - New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC
JF  - Environmental toxicology
VL  - 32
IS  - 2
SP  - 569-580
EP  - 569-580
PB  - WILEY-BLACKWELL
SN  - 15204081
KW  - ethylhexyl methoxycinnamate
KW  - trans/cis isomerisation
KW  - genotoxicity
KW  - probabilistic risk assessment
KW  - QIVIVE
UR  - http://onlinelibrary.wiley.com/doi/10.1002/tox.22260/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/tox.22260/abstract
N2  - Ethylhexyl methoxycinnamate (EHMC) is a widely used UV filter present in a large number of personal care products (PCPs). Under normal conditions, EHMC occurs in a mixture of two isomers: trans-EHMC and cis-EHMC in a ratio of 99:1. When exposed to sunlight, the trans isomer is transformed to the less stable cis isomer and the efficiency of the UV filter is reduced. To date, the toxicological effects of the cis-EHMC isomer remain largely unknown. We developed a completely new method for preparing cis-EHMC. An EHMC technical mixture was irradiated using a UV lamp and 98% pure cis-EHMC was isolated from the irradiated solution using column chromatography. The genotoxic effects of the isolated cis-EHMC isomer and the nonirradiated trans-EHMC were subsequently measured using two bioassays (SOS chromotest and UmuC test). In the case of trans-EHMC, significant genotoxicity was observed using both bioassays at the highest concentrations (0.5 - 4 mgmL(-1)). In the case of cis-EHMC, significant genotoxicity was only detected using the UmuC test at concentrations of 0.25 - 1 mgmL(-1). Based on these results, the NOEC was calculated for both cis- and trans-EHMC, 0.038 and 0.064 mgmL(-1), respectively. Risk assessment of dermal, oral and inhalation exposure to PCPs containing EHMC was carried out for a female population using probabilistic simulation and by using Quantitative in vitro to in vivo extrapolation (QIVIVE). The risk of cis-EHMC was found to be approximate to 1.7 times greater than trans-EHMC. In the case of cis-EHMC, a hazard index of 1 was exceeded in the 92nd percentile. Based on the observed differences between the isomers, EHMC application in PCPs requires detailed reassessment. Further exploration of the toxicological effects and properties of cis-EHMC is needed in order to correctly predict risks posed to humans and the environment.
ER  -

SHARMA, Anežka, Katarína BÁNYIOVÁ, Jaromír LITERÁK and Pavel ČUPR. New probabilistic risk assessment of ethylhexyl methoxycinnamate: Comparing the genotoxic effects of trans- and cis-EHMC. \textit{Environmental toxicology}. Hoboken, USA: WILEY-BLACKWELL, 2017, vol.~32, No~2, p.~569-580. ISSN~1520-4081. Available from: https://dx.doi.org/10.1002/tox.22260.

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