Aggregate Size Dependence of Amyloid Adsorption onto Charged
Interfaces

J 2018

Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces

TESEI, Giulio, Erik HELLSTRAND, Kalyani SANAGAVARAPU, Sara LINSE, Emma SPARR et. al.

Basic information

Original name

Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces

Authors

TESEI, Giulio (752 Sweden), Erik HELLSTRAND (752 Sweden), Kalyani SANAGAVARAPU (752 Sweden), Sara LINSE (752 Sweden), Emma SPARR (752 Sweden), Robert VÁCHA (203 Czech Republic, guarantor, belonging to the institution) and Mikael LUND (752 Sweden)

Edition

Langmuir, WASHINGTON, AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2018, 0743-7463

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.683

RIV identification code

RIV/00216224:14740/18:00100839

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acs.langmuir.7b03155

UT WoS

000424070400005

Keywords in English

QUARTZ-CRYSTAL MICROBALANCE; ATOMIC-RESOLUTION STRUCTURE; POISSON-BOLTZMANN THEORY; AIR-WATER-INTERFACE; ALPHA-SYNUCLEIN; MONTE-CARLO; STRUCTURAL BASIS; LIPID-MEMBRANES; BETA-PEPTIDE; FIBRILS

Abstract

V originále

Amyloid aggregates are associated with a range of human neuro-degenerative disorders, and it has been shown that neurotoxicity is dependent on aggregate size. Combining molecular simulation with analytical theory, a predictive model is proposed for the adsorption of amyloid aggregates onto oppositely charged surfaces, where the interaction is governed by an interplay between electrostatic attraction and entropic repulsion. Predictions are experimentally validated against quartz crystal microbalance-dissipation experiments of amyloid beta peptides and fragmented fibrils in the presence of a supported lipid bilayer. Assuming amyloids as rigid, elongated particles, we observe nonmonotonic trends for the extent of adsorption with respect to aggregate size and preferential adsorption of smaller aggregates over larger ones. Our findings describe a general phenomenon with implications for stiff polyions and rodlike particles that are electrostatically attracted to a surface.

Links

ED3.2.00/08.0144, research and development project

Name: CERIT Scientific Cloud

GA14-12598S, research and development project

Name: Samouspořádané systémy amfifilních peptiů (Acronym: SAAP)

Investor: Czech Science Foundation

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

TESEI, Giulio, Erik HELLSTRAND, Kalyani SANAGAVARAPU, Sara LINSE, Emma SPARR, Robert VÁCHA and Mikael LUND. Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces. Langmuir. WASHINGTON: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2018, vol. 34, No 4, p. 1266-1273. ISSN 0743-7463. Available from: https://dx.doi.org/10.1021/acs.langmuir.7b03155.

@article{1409325,
   author = {Tesei, Giulio and Hellstrand, Erik and Sanagavarapu, Kalyani and Linse, Sara and Sparr, Emma and Vácha, Robert and Lund, Mikael},
   article_location = {WASHINGTON},
   article_number = {4},
   doi = {http://dx.doi.org/10.1021/acs.langmuir.7b03155},
   keywords = {QUARTZ-CRYSTAL MICROBALANCE; ATOMIC-RESOLUTION STRUCTURE; POISSON-BOLTZMANN THEORY; AIR-WATER-INTERFACE; ALPHA-SYNUCLEIN; MONTE-CARLO; STRUCTURAL BASIS; LIPID-MEMBRANES; BETA-PEPTIDE; FIBRILS},
   language = {eng},
   issn = {0743-7463},
   journal = {Langmuir},
   title = {Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces},
   url = {https://pubs.acs.org/doi/10.1021/acs.langmuir.7b03155},
   volume = {34},
   year = {2018}
}

TY  - JOUR
ID  - 1409325
AU  - Tesei, Giulio - Hellstrand, Erik - Sanagavarapu, Kalyani - Linse, Sara - Sparr, Emma - Vácha, Robert - Lund, Mikael
PY  - 2018
TI  - Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces
JF  - Langmuir
VL  - 34
IS  - 4
SP  - 1266-1273
EP  - 1266-1273
PB  - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN  - 07437463
KW  - QUARTZ-CRYSTAL MICROBALANCE
KW  - ATOMIC-RESOLUTION STRUCTURE
KW  - POISSON-BOLTZMANN THEORY
KW  - AIR-WATER-INTERFACE
KW  - ALPHA-SYNUCLEIN
KW  - MONTE-CARLO
KW  - STRUCTURAL BASIS
KW  - LIPID-MEMBRANES
KW  - BETA-PEPTIDE
KW  - FIBRILS
UR  - https://pubs.acs.org/doi/10.1021/acs.langmuir.7b03155
N2  - Amyloid aggregates are associated with a range of human neuro-degenerative disorders, and it has been shown that neurotoxicity is dependent on aggregate size. Combining molecular simulation with analytical theory, a predictive model is proposed for the adsorption of amyloid aggregates onto oppositely charged surfaces, where the interaction is governed by an interplay between electrostatic attraction and entropic repulsion. Predictions are experimentally validated against quartz crystal microbalance-dissipation experiments of amyloid beta peptides and fragmented fibrils in the presence of a supported lipid bilayer. Assuming amyloids as rigid, elongated particles, we observe nonmonotonic trends for the extent of adsorption with respect to aggregate size and preferential adsorption of smaller aggregates over larger ones. Our findings describe a general phenomenon with implications for stiff polyions and rodlike particles that are electrostatically attracted to a surface.
ER  -

TESEI, Giulio, Erik HELLSTRAND, Kalyani SANAGAVARAPU, Sara LINSE, Emma SPARR, Robert VÁCHA and Mikael LUND. Aggregate Size Dependence of Amyloid Adsorption onto Charged Interfaces. \textit{Langmuir}. WASHINGTON: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2018, vol.~34, No~4, p.~1266-1273. ISSN~0743-7463. Available from: https://dx.doi.org/10.1021/acs.langmuir.7b03155.

Detailed Information on Publication Record