Reprogramming of peripheral blood cells into hiPSCs as a source
of endothelial cells

a 2018

Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ et. al.

Basic information

Original name

Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells

Authors

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA and Irena KRONTORÁD KOUTNÁ

Edition

SY-Stem symposium 2018, 2018

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10601 Cell biology

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

ABSTRACT BOOKLET 2018

Organization unit

Faculty of Informatics

Keywords (in Czech)

buněčná reprogramace, endotheliální diferenciace

Keywords in English

cellular reprogramming, endothelial differentiation

Změněno: 2/3/2018 10:41, Mgr. Pavel Šimara, Ph.D.

Abstract

V originále

Recent advances in human induced pluripotent stem cell (hiPSC) research have opened avenues to generate unlimited numbers of endothelial cells (ECs) from easily accessible cell sources, such as the peripheral blood. We reprogrammed peripheral blood mononuclear cells (PBMCs), human umbilical vein endothelial cells (HUVECs) and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. hiPSC-derived ECs were compared to HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts both phenotypically and functionally. An increased number of DNA double-strand breaks (γH2AX) upon reprogramming was observed. However, differentiation into ECs restored a normal number of γH2AX foci. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

NV16-31501A, research and development project

Name: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA and Irena KRONTORÁD KOUTNÁ. Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells. In SY-Stem symposium 2018. 2018.

@proceedings{1409570,
   author = {Šimara, Pavel and Tesařová, Lenka and Řeháková, Daniela and Farkaš, Šimon and Šalingová, Barbara and Kutálková, Kateřina and Vavrečková, Eva and Matula, Pavel and Matula, Petr and Krontorád Koutná, Irena},
   booktitle = {SY-Stem symposium 2018},
   keywords = {cellular reprogramming, endothelial differentiation},
   language = {eng},
   title = {Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells},
   url = {https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/},
   year = {2018}
}

TY  - CONF
ID  - 1409570
AU  - Šimara, Pavel - Tesařová, Lenka - Řeháková, Daniela - Farkaš, Šimon - Šalingová, Barbara - Kutálková, Kateřina - Vavrečková, Eva - Matula, Pavel - Matula, Petr - Krontorád Koutná, Irena
PY  - 2018
TI  - Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells
KW  - cellular reprogramming, endothelial differentiation
UR  - https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/
N2  - Recent advances in human induced pluripotent stem cell (hiPSC) research have opened avenues to generate unlimited numbers of endothelial cells (ECs) from easily accessible cell sources, such as the peripheral blood. We reprogrammed peripheral blood mononuclear cells (PBMCs), human umbilical vein endothelial cells (HUVECs) and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. hiPSC-derived ECs were compared to HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts both phenotypically and functionally. An increased number of DNA double-strand breaks (γH2AX) upon reprogramming was observed. However, differentiation into ECs restored a normal number of γH2AX foci. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage.
ER  -

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