Diverse responses of wild and cultivated tomato to BABA,
oligandrin and Oidium neolycopersici infection

J 2017

Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection

SATKOVÁ, Pavla, Tomáš STARÝ, Veronika PLEŠKOVÁ, Martina ZAPLETALOVÁ, Tomáš KAŠPAROVSKÝ et. al.

Základní údaje

Originální název

Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection

Autoři

SATKOVÁ, Pavla (203 Česká republika), Tomáš STARÝ (203 Česká republika, domácí), Veronika PLEŠKOVÁ (203 Česká republika, domácí), Martina ZAPLETALOVÁ (203 Česká republika, domácí), Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí), Lucie CINCALOVA-KUBIENOVA (203 Česká republika), Lenka LUHOVÁ (203 Česká republika), Barbora MIESLEROVA (203 Česká republika), Jaromír MIKULIK (203 Česká republika), Jan LOCHMAN (203 Česká republika, garant, domácí) a Marek PETŘIVALSKÝ (203 Česká republika)

Vydání

Annals of Botany, Oxford, Oxford University Press, 2017, 0305-7364

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.646

Kód RIV

RIV/00216224:14310/17:00095531

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/aob/mcw188

UT WoS

000400982600012

Klíčová slova anglicky

BABA; defence genes; ethylene; Oidium neolycopersici; oligandrin; powdery mildew; resistance; Solanum lycopersicum; Solanum habrochaites; tomato

Štítky

NZ, rivok

Změněno: 1. 4. 2018 10:12, Ing. Nicole Zrilić

Anotace

V originále

Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen. Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent beta-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites. Key Results Differences in basal expression levels of defensins, germins, beta-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype. Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin- and BABA-induced resistance involved different signalling pathways, with BABA-treated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.

Návaznosti

GAP501/12/0590, projekt VaV

Název: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Grantová agentura ČR, Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Citovat

SATKOVÁ, Pavla, Tomáš STARÝ, Veronika PLEŠKOVÁ, Martina ZAPLETALOVÁ, Tomáš KAŠPAROVSKÝ, Lucie CINCALOVA-KUBIENOVA, Lenka LUHOVÁ, Barbora MIESLEROVA, Jaromír MIKULIK, Jan LOCHMAN a Marek PETŘIVALSKÝ. Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection. Annals of Botany. Oxford: Oxford University Press, 2017, roč. 119, č. 5, s. 829-840. ISSN 0305-7364. Dostupné z: https://dx.doi.org/10.1093/aob/mcw188.

@article{1409616,
   author = {Satková, Pavla and Starý, Tomáš and Plešková, Veronika and Zapletalová, Martina and Kašparovský, Tomáš and CincalovaandKubienova, Lucie and Luhová, Lenka and Mieslerova, Barbora and Mikulik, Jaromír and Lochman, Jan and Petřivalský, Marek},
   article_location = {Oxford},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/aob/mcw188},
   keywords = {BABA; defence genes; ethylene; Oidium neolycopersici; oligandrin; powdery mildew; resistance; Solanum lycopersicum; Solanum habrochaites; tomato},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection},
   volume = {119},
   year = {2017}
}

TY  - JOUR
ID  - 1409616
AU  - Satková, Pavla - Starý, Tomáš - Plešková, Veronika - Zapletalová, Martina - Kašparovský, Tomáš - Cincalova-Kubienova, Lucie - Luhová, Lenka - Mieslerova, Barbora - Mikulik, Jaromír - Lochman, Jan - Petřivalský, Marek
PY  - 2017
TI  - Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection
JF  - Annals of Botany
VL  - 119
IS  - 5
SP  - 829-840
EP  - 829-840
PB  - Oxford University Press
SN  - 03057364
KW  - BABA
KW  - defence genes
KW  - ethylene
KW  - Oidium neolycopersici
KW  - oligandrin
KW  - powdery mildew
KW  - resistance
KW  - Solanum lycopersicum
KW  - Solanum habrochaites
KW  - tomato
N2  - Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen. Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent beta-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites. Key Results Differences in basal expression levels of defensins, germins, beta-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype. Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin- and BABA-induced resistance involved different signalling pathways, with BABA-treated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.
ER  -

SATKOVÁ, Pavla, Tomáš STARÝ, Veronika PLEŠKOVÁ, Martina ZAPLETALOVÁ, Tomáš KAŠPAROVSKÝ, Lucie CINCALOVA-KUBIENOVA, Lenka LUHOVÁ, Barbora MIESLEROVA, Jaromír MIKULIK, Jan LOCHMAN a Marek PETŘIVALSKÝ. Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection. \textit{Annals of Botany}. Oxford: Oxford University Press, 2017, roč.~119, č.~5, s.~829-840. ISSN~0305-7364. Dostupné z: https://dx.doi.org/10.1093/aob/mcw188.

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