Detailed Information on Publication Record
2017
Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection
SATKOVÁ, Pavla, Tomáš STARÝ, Veronika PLEŠKOVÁ, Martina ZAPLETALOVÁ, Tomáš KAŠPAROVSKÝ et. al.Basic information
Original name
Diverse responses of wild and cultivated tomato to BABA, oligandrin and Oidium neolycopersici infection
Authors
SATKOVÁ, Pavla (203 Czech Republic), Tomáš STARÝ (203 Czech Republic, belonging to the institution), Veronika PLEŠKOVÁ (203 Czech Republic, belonging to the institution), Martina ZAPLETALOVÁ (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Lucie CINCALOVA-KUBIENOVA (203 Czech Republic), Lenka LUHOVÁ (203 Czech Republic), Barbora MIESLEROVA (203 Czech Republic), Jaromír MIKULIK (203 Czech Republic), Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution) and Marek PETŘIVALSKÝ (203 Czech Republic)
Edition
Annals of Botany, Oxford, Oxford University Press, 2017, 0305-7364
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.646
RIV identification code
RIV/00216224:14310/17:00095531
Organization unit
Faculty of Science
UT WoS
000400982600012
Keywords in English
BABA; defence genes; ethylene; Oidium neolycopersici; oligandrin; powdery mildew; resistance; Solanum lycopersicum; Solanum habrochaites; tomato
Změněno: 1/4/2018 10:12, Ing. Nicole Zrilić
Abstract
V originále
Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen. Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent beta-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites. Key Results Differences in basal expression levels of defensins, germins, beta-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype. Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin- and BABA-induced resistance involved different signalling pathways, with BABA-treated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.
Links
| GAP501/12/0590, research and development project |
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