MAMP (microbe-associated molecular pattern)-induced changes in
plasma membrane-associated proteins

J 2017

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ et. al.

Basic information

Original name

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

Authors

UHLÍKOVÁ, Hana (203 Czech Republic, belonging to the institution), Martin SOLANSKÝ (203 Czech Republic, belonging to the institution), Vendula HRDINOVÁ (203 Czech Republic, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Plant Physiology, JENA, ELSEVIER GMBH, 2017, 0176-1617

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.833

RIV identification code

RIV/00216224:14310/17:00095532

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jplph.2016.12.005

UT WoS

000397471200006

Keywords in English

Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase

Abstract

V originále

Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.

Links

GAP501/12/0590, research and development project

Name: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ, Jan HEJÁTKO and Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. Journal of Plant Physiology. JENA: ELSEVIER GMBH, 2017, vol. 210, March, p. 51-57. ISSN 0176-1617. Available from: https://dx.doi.org/10.1016/j.jplph.2016.12.005.

@article{1409620,
   author = {Uhlíková, Hana and Solanský, Martin and Hrdinová, Vendula and Šedo, Ondrej and Kašparovský, Tomáš and Hejátko, Jan and Lochman, Jan},
   article_location = {JENA},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.jplph.2016.12.005},
   keywords = {Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase},
   language = {eng},
   issn = {0176-1617},
   journal = {Journal of Plant Physiology},
   note = {Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.},
   title = {MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins},
   url = {http://dx.doi.org/10.1016/j.jplph.2016.12.005},
   volume = {210},
   year = {2017}
}

TY  - JOUR
ID  - 1409620
AU  - Uhlíková, Hana - Solanský, Martin - Hrdinová, Vendula - Šedo, Ondrej - Kašparovský, Tomáš - Hejátko, Jan - Lochman, Jan
PY  - 2017
TI  - MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins
JF  - Journal of Plant Physiology
VL  - 210
IS  - March
SP  - 51-57
EP  - 51-57
PB  - ELSEVIER GMBH
SN  - 01761617
N1  - Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.
KW  - Cryptogein
KW  - Rab8
KW  - Resistance
KW  - ATPase
KW  - NADP-malic enzyme
KW  - Glutamine synthetase
UR  - http://dx.doi.org/10.1016/j.jplph.2016.12.005
N2  - Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.
ER  -

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ, Jan HEJÁTKO and Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. \textit{Journal of Plant Physiology}. JENA: ELSEVIER GMBH, 2017, vol.~210, March, p.~51-57. ISSN~0176-1617. Available from: https://dx.doi.org/10.1016/j.jplph.2016.12.005.

Detailed Information on Publication Record