J 2018

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

DŽATKO, Šimon, Michaela KRAFČÍKOVÁ, R. HANSEL-HERTSCH, T. FESSL, Radovan FIALA et. al.

Basic information

Original name

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Authors

DŽATKO, Šimon (703 Slovakia, belonging to the institution), Michaela KRAFČÍKOVÁ (703 Slovakia, belonging to the institution), R. HANSEL-HERTSCH (826 United Kingdom of Great Britain and Northern Ireland), T. FESSL (203 Czech Republic), Radovan FIALA (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Daniel KRAFČÍK (703 Slovakia, belonging to the institution), J.L. MERGNY (250 France), Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution) and Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Angewandte Chemie International Edition, WEINHEIM (GERMANY), Verlag Chemie, 2018, 1433-7851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 12.257

RIV identification code

RIV/00216224:14740/18:00100847

Organization unit

Central European Institute of Technology

DOI

UT WoS

000424879400024

Keywords in English

DNA; i-motifs; in-cell NMR spectroscopy; structural biology

Tags

Tags

International impact, Reviewed
Změněno: 13/3/2019 13:16, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.

Links

GA16-10504S, research and development project
Name: Charakterizace struktury nukleových kyselin v komplexním prostředí živých buněk pomocí vysoce rozlišené NMR spektroskopie
Investor: Czech Science Foundation
LM2015043, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LM2015062, research and development project
Name: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR
LM2015064, research and development project
Name: Český národní uzel Evropské infrastruktury pro translační medicínu (Acronym: EATRIS-ERIC-CZ)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
2535, interní kód MU
Name: Cellular Structural Biology of non-B DNA Motifs in Human Genome
Investor: EMBO (European Molecular Biology Organization)
653706, interní kód MU
Name: iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research (Acronym: iNEXT)
Investor: European Union, iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research, RI Research Infrastructures (Excellent Science)