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@article{1410037,
author = {Seifertová, Marta and Čechová, Eliška and Llansola, Marta and Felipo, Vicente and Vykoukalová, Martina and Kočan, Anton},
article_location = {HEIDELBERG},
article_number = {25},
doi = {http://dx.doi.org/10.1007/s00216-017-0533-1},
keywords = {Animal tissues; Insecticides; Mini-extractor; Neurodevelopmental toxicity; Tissue extract clean-up},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical chemistry},
title = {Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor},
url = {https://link.springer.com/article/10.1007%2Fs00216-017-0533-1},
volume = {409},
year = {2017}
}
TY - JOUR
ID - 1410037
AU - Seifertová, Marta - Čechová, Eliška - Llansola, Marta - Felipo, Vicente - Vykoukalová, Martina - Kočan, Anton
PY - 2017
TI - Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor
JF - Analytical and Bioanalytical chemistry
VL - 409
IS - 25
SP - 6015-6026
EP - 6015-6026
PB - SPRINGER HEIDELBERG
SN - 16182642
KW - Animal tissues
KW - Insecticides
KW - Mini-extractor
KW - Neurodevelopmental toxicity
KW - Tissue extract clean-up
UR - https://link.springer.com/article/10.1007%2Fs00216-017-0533-1
L2 - https://link.springer.com/article/10.1007%2Fs00216-017-0533-1
N2 - We developed a simple analytical method for the simultaneous determination of representatives of various groups of neurotoxic insecticides (carbaryl, chlorpyrifos, cypermethrin, and alpha-endosulfan and beta-endosulfan and their metabolite endosulfan sulfate) in limited amounts of animal tissues containing different amounts of lipids. Selected tissues (rodent fat, liver, and brain) were extracted in a special in-house-designed mini-extractor constructed on the basis of the Soxhlet and Twisselmann extractors. A dried tissue sample placed in a small cartridge was extracted, while the nascent extract was simultaneously filtered through a layer of sodium sulfate. The extraction was followed by combined clean-up, including gel permeation chromatography (in case of high lipid content), ultrasonication, and solid-phase extraction chromatography using C-18 on silica and aluminum oxide. Gas chromatography coupled with high-resolution mass spectrometry was used for analyte separation, detection, and quantification. Average recoveries for individual insecticides ranged from 82 to 111%. Expanded measurement uncertainties were generally lower than 35%. The developed method was successfully applied to rat tissue samples obtained from an animal model dealing with insecticide exposure during brain development. This method may also be applied to the analytical treatment of small amounts of various types of animal and human tissue samples. A significant advantage achieved using this method is high sample throughput due to the simultaneous treatment of many samples.
ER -
SEIFERTOVÁ, Marta, Eliška ČECHOVÁ, Marta LLANSOLA, Vicente FELIPO, Martina VYKOUKALOVÁ and Anton KOČAN. Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol.~409, No~25, p.~6015-6026. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-017-0533-1.
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