Determination of selected neurotoxic insecticides in small
amounts of animal tissue utilizing a newly constructed
mini-extractor

J 2017

Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor

SEIFERTOVÁ, Marta, Eliška ČECHOVÁ, Marta LLANSOLA, Vicente FELIPO, Martina VYKOUKALOVÁ et. al.

Basic information

Original name

Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor

Authors

SEIFERTOVÁ, Marta (203 Czech Republic, belonging to the institution), Eliška ČECHOVÁ (203 Czech Republic, belonging to the institution), Marta LLANSOLA (724 Spain), Vicente FELIPO (724 Spain), Martina VYKOUKALOVÁ (203 Czech Republic, belonging to the institution) and Anton KOČAN (703 Slovakia, guarantor, belonging to the institution)

Edition

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.307

RIV identification code

RIV/00216224:14310/17:00100205

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00216-017-0533-1

UT WoS

000410798600020

Keywords in English

Animal tissues; Insecticides; Mini-extractor; Neurodevelopmental toxicity; Tissue extract clean-up

Abstract

V originále

We developed a simple analytical method for the simultaneous determination of representatives of various groups of neurotoxic insecticides (carbaryl, chlorpyrifos, cypermethrin, and alpha-endosulfan and beta-endosulfan and their metabolite endosulfan sulfate) in limited amounts of animal tissues containing different amounts of lipids. Selected tissues (rodent fat, liver, and brain) were extracted in a special in-house-designed mini-extractor constructed on the basis of the Soxhlet and Twisselmann extractors. A dried tissue sample placed in a small cartridge was extracted, while the nascent extract was simultaneously filtered through a layer of sodium sulfate. The extraction was followed by combined clean-up, including gel permeation chromatography (in case of high lipid content), ultrasonication, and solid-phase extraction chromatography using C-18 on silica and aluminum oxide. Gas chromatography coupled with high-resolution mass spectrometry was used for analyte separation, detection, and quantification. Average recoveries for individual insecticides ranged from 82 to 111%. Expanded measurement uncertainties were generally lower than 35%. The developed method was successfully applied to rat tissue samples obtained from an animal model dealing with insecticide exposure during brain development. This method may also be applied to the analytical treatment of small amounts of various types of animal and human tissue samples. A significant advantage achieved using this method is high sample throughput due to the simultaneous treatment of many samples.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

282957, interní kód MU

Name: DENAMIC - Developmental neurotoxicity assessment of mixtures in children (Acronym: DENAMIC)

Investor: European Union, Cooperation

Citovat

SEIFERTOVÁ, Marta, Eliška ČECHOVÁ, Marta LLANSOLA, Vicente FELIPO, Martina VYKOUKALOVÁ and Anton KOČAN. Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol. 409, No 25, p. 6015-6026. ISSN 1618-2642. Available from: https://dx.doi.org/10.1007/s00216-017-0533-1.

@article{1410037,
   author = {Seifertová, Marta and Čechová, Eliška and Llansola, Marta and Felipo, Vicente and Vykoukalová, Martina and Kočan, Anton},
   article_location = {HEIDELBERG},
   article_number = {25},
   doi = {http://dx.doi.org/10.1007/s00216-017-0533-1},
   keywords = {Animal tissues; Insecticides; Mini-extractor; Neurodevelopmental toxicity; Tissue extract clean-up},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor},
   url = {https://link.springer.com/article/10.1007%2Fs00216-017-0533-1},
   volume = {409},
   year = {2017}
}

TY  - JOUR
ID  - 1410037
AU  - Seifertová, Marta - Čechová, Eliška - Llansola, Marta - Felipo, Vicente - Vykoukalová, Martina - Kočan, Anton
PY  - 2017
TI  - Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor
JF  - Analytical and Bioanalytical chemistry
VL  - 409
IS  - 25
SP  - 6015-6026
EP  - 6015-6026
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Animal tissues
KW  - Insecticides
KW  - Mini-extractor
KW  - Neurodevelopmental toxicity
KW  - Tissue extract clean-up
UR  - https://link.springer.com/article/10.1007%2Fs00216-017-0533-1
L2  - https://link.springer.com/article/10.1007%2Fs00216-017-0533-1
N2  - We developed a simple analytical method for the simultaneous determination of representatives of various groups of neurotoxic insecticides (carbaryl, chlorpyrifos, cypermethrin, and alpha-endosulfan and beta-endosulfan and their metabolite endosulfan sulfate) in limited amounts of animal tissues containing different amounts of lipids. Selected tissues (rodent fat, liver, and brain) were extracted in a special in-house-designed mini-extractor constructed on the basis of the Soxhlet and Twisselmann extractors. A dried tissue sample placed in a small cartridge was extracted, while the nascent extract was simultaneously filtered through a layer of sodium sulfate. The extraction was followed by combined clean-up, including gel permeation chromatography (in case of high lipid content), ultrasonication, and solid-phase extraction chromatography using C-18 on silica and aluminum oxide. Gas chromatography coupled with high-resolution mass spectrometry was used for analyte separation, detection, and quantification. Average recoveries for individual insecticides ranged from 82 to 111%. Expanded measurement uncertainties were generally lower than 35%. The developed method was successfully applied to rat tissue samples obtained from an animal model dealing with insecticide exposure during brain development. This method may also be applied to the analytical treatment of small amounts of various types of animal and human tissue samples. A significant advantage achieved using this method is high sample throughput due to the simultaneous treatment of many samples.
ER  -

SEIFERTOVÁ, Marta, Eliška ČECHOVÁ, Marta LLANSOLA, Vicente FELIPO, Martina VYKOUKALOVÁ and Anton KOČAN. Determination of selected neurotoxic insecticides in small amounts of animal tissue utilizing a newly constructed mini-extractor. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol.~409, No~25, p.~6015-6026. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-017-0533-1.

Detailed Information on Publication Record