DNA interaction with platinum-based cytostatics revealed by DNA
sequencing

J 2017

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY et. al.

Basic information

Original name

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Authors

SMERKOVA, Kristyna (203 Czech Republic), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Markéta VACULOVIČOVÁ (203 Czech Republic), Jindrich KYNICKY (203 Czech Republic), Martin BRTNICKY (203 Czech Republic), Tomas ECKSCHLAGER (203 Czech Republic), Marie STIBOROVA (203 Czech Republic), Jaromir HUBALEK (203 Czech Republic) and Vojtěch ADAM (203 Czech Republic, guarantor)

Edition

Analytical Biochemistry, San Diego, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, 0003-2697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 2.275

RIV identification code

RIV/00216224:14310/17:00100210

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ab.2017.09.018

UT WoS

000417117000004

Keywords in English

DNA; Platinum based cytostatics; Sequencing; Mass spectrometry

Abstract

V originále

The main mechanism of action of platinum-based cytostatic drugs cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 mu g/mL), oxaliplatin (5.23 mu g/mL), and carboplatin (71.11 mu g/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 mu g/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of mu g per 5 mu g of DNA.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY, Tomas ECKSCHLAGER, Marie STIBOROVA, Jaromir HUBALEK and Vojtěch ADAM. DNA interaction with platinum-based cytostatics revealed by DNA sequencing. Analytical Biochemistry. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, vol. 539, DEC, p. 22-28. ISSN 0003-2697. Available from: https://dx.doi.org/10.1016/j.ab.2017.09.018.

@article{1410075,
   author = {Smerkova, Kristyna and Vaculovič, Tomáš and Vaculovičová, Markéta and Kynicky, Jindrich and Brtnicky, Martin and Eckschlager, Tomas and Stiborova, Marie and Hubalek, Jaromir and Adam, Vojtěch},
   article_location = {San Diego},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.ab.2017.09.018},
   keywords = {DNA; Platinum based cytostatics; Sequencing; Mass spectrometry},
   language = {eng},
   issn = {0003-2697},
   journal = {Analytical Biochemistry},
   title = {DNA interaction with platinum-based cytostatics revealed by DNA sequencing},
   url = {https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub},
   volume = {539},
   year = {2017}
}

TY  - JOUR
ID  - 1410075
AU  - Smerkova, Kristyna - Vaculovič, Tomáš - Vaculovičová, Markéta - Kynicky, Jindrich - Brtnicky, Martin - Eckschlager, Tomas - Stiborova, Marie - Hubalek, Jaromir - Adam, Vojtěch
PY  - 2017
TI  - DNA interaction with platinum-based cytostatics revealed by DNA sequencing
JF  - Analytical Biochemistry
VL  - 539
IS  - DEC
SP  - 22-28
EP  - 22-28
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00032697
KW  - DNA
KW  - Platinum based cytostatics
KW  - Sequencing
KW  - Mass spectrometry
UR  - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub
N2  - The main mechanism of action of platinum-based cytostatic drugs cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 mu g/mL), oxaliplatin (5.23 mu g/mL), and carboplatin (71.11 mu g/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 mu g/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of mu g per 5 mu g of DNA.
ER  -

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY, Tomas ECKSCHLAGER, Marie STIBOROVA, Jaromir HUBALEK and Vojtěch ADAM. DNA interaction with platinum-based cytostatics revealed by DNA sequencing. \textit{Analytical Biochemistry}. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, vol.~539, DEC, p.~22-28. ISSN~0003-2697. Available from: https://dx.doi.org/10.1016/j.ab.2017.09.018.

Detailed Information on Publication Record