| MASNER, Petr, Barbora JAVŮRKOVÁ and Luděk BLÁHA. Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer. Environmental Science and Pollution Research. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol. 24, No 4, p. 3748-3758. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-016-8096-9. |
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@article{1410107,
author = {Masner, Petr and Javůrková, Barbora and Bláha, Luděk},
article_location = {HEIDELBERG},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s11356-016-8096-9},
keywords = {Photorhabdus luminescens; Vibrio fisheri; Whole effluent testing (WET); In situ testing; Portable biotest},
language = {eng},
issn = {0944-1344},
journal = {Environmental Science and Pollution Research},
title = {Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer},
url = {https://link.springer.com/article/10.1007%2Fs11356-016-8096-9},
volume = {24},
year = {2017}
}
TY - JOUR
ID - 1410107
AU - Masner, Petr - Javůrková, Barbora - Bláha, Luděk
PY - 2017
TI - Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer
JF - Environmental Science and Pollution Research
VL - 24
IS - 4
SP - 3748-3758
EP - 3748-3758
PB - SPRINGER HEIDELBERG
SN - 09441344
KW - Photorhabdus luminescens
KW - Vibrio fisheri
KW - Whole effluent testing (WET)
KW - In situ testing
KW - Portable biotest
UR - https://link.springer.com/article/10.1007%2Fs11356-016-8096-9
L2 - https://link.springer.com/article/10.1007%2Fs11356-016-8096-9
N2 - The present study demonstrates development of a rapid testing protocol based on a small portable luminometer using flash kinetic assessment of bacterial bioluminescence. The laboratory comparisons based on six model organic toxicants and two metals showed significant correlations between responses of freshwater bacteria Photorhabdus luminescens and standard marine bacterial species Vibrio fisheri. While P. luminescens was less sensitive in standard arrangements, the responses of both organisms were comparable in the newly introduced portable luminometer setup. The applicability and reproducibility of the portable luminometer protocol was further demonstrated in the assessment of 43 European wastewater effluents that were simultaneously tested for toxicity and analysed for 150 organic and 20 inorganic contaminants grouped into 13 major chemical classes. Clear association between the toxic responses in both compared bacterial species and the elevated levels of inorganic compounds (toxic metals), chlorophenols and benzotriazole anticorrosives was observed. The new protocol with a portable luminometer provides a fast (30 s) response and may be used as a tool for rapid in situ toxicity evaluation of freshwater environmental samples such as effluents.
ER -
MASNER, Petr, Barbora JAVŮRKOVÁ and Luděk BLÁHA. Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer. \textit{Environmental Science and Pollution Research}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol.~24, No~4, p.~3748-3758. ISSN~0944-1344. Available from: https://dx.doi.org/10.1007/s11356-016-8096-9.
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