Phytoestrogens and sterols in waters with cyanobacterial blooms
- Analytical methods and estrogenic potencies

J 2017

Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK et. al.

Basic information

Original name

Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

Authors

PROCHÁZKOVÁ, Tereza (203 Czech Republic, belonging to the institution), Eliška SYCHROVÁ (203 Czech Republic, belonging to the institution), Barbora JAVŮRKOVÁ (203 Czech Republic, belonging to the institution), Jaroslava VEČERKOVÁ (203 Czech Republic, belonging to the institution), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution), Olga LEPSOVA-SKACELOVA (203 Czech Republic), Luděk BLÁHA (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemosphere, Oxford, PERGAMON-ELSEVIER SCIENCE, 2017, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.427

RIV identification code

RIV/00216224:14310/17:00100222

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chemosphere.2016.12.006

UT WoS

000392897100013

Keywords in English

Flavonoid; Sterol; Relative estrogenic potency; Estrogenicity; Reservoir

Abstract

V originále

Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

603437, interní kód MU

Name: SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management (Acronym: SOLUTIONS)

Investor: European Union, Cooperation

Citovat

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK, Olga LEPSOVA-SKACELOVA, Luděk BLÁHA and Klára HILSCHEROVÁ. Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies. Chemosphere. Oxford: PERGAMON-ELSEVIER SCIENCE, 2017, vol. 170, March, p. 104-112. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2016.12.006.

@article{1410111,
   author = {Procházková, Tereza and Sychrová, Eliška and Javůrková, Barbora and Večerková, Jaroslava and Kohoutek, Jiří and LepsovaandSkacelova, Olga and Bláha, Luděk and Hilscherová, Klára},
   article_location = {Oxford},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.12.006},
   keywords = {Flavonoid; Sterol; Relative estrogenic potency; Estrogenicity; Reservoir},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub},
   volume = {170},
   year = {2017}
}

TY  - JOUR
ID  - 1410111
AU  - Procházková, Tereza - Sychrová, Eliška - Javůrková, Barbora - Večerková, Jaroslava - Kohoutek, Jiří - Lepsova-Skacelova, Olga - Bláha, Luděk - Hilscherová, Klára
PY  - 2017
TI  - Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies
JF  - Chemosphere
VL  - 170
IS  - March
SP  - 104-112
EP  - 104-112
PB  - PERGAMON-ELSEVIER SCIENCE
SN  - 00456535
KW  - Flavonoid
KW  - Sterol
KW  - Relative estrogenic potency
KW  - Estrogenicity
KW  - Reservoir
UR  - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub
N2  - Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.
ER  -

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK, Olga LEPSOVA-SKACELOVA, Luděk BLÁHA and Klára HILSCHEROVÁ. Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies. \textit{Chemosphere}. Oxford: PERGAMON-ELSEVIER SCIENCE, 2017, vol.~170, March, p.~104-112. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2016.12.006.

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