Replication of ribosomal DNA in Arabidopsis occurs both inside
and outside the nucleolus during S phase progression

J 2018

Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression

DVOŘÁČKOVÁ, Martina; B. RAPOSO; Petr MATULA; J. FUCHS; V. SCHUBERT et al.

Základní údaje

Originální název

Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression

Autoři

DVOŘÁČKOVÁ, Martina ; B. RAPOSO; Petr MATULA; J. FUCHS; V. SCHUBERT; Vratislav PEŠKA; B. DESVOYES; C. GUTIERREZ a Jiří FAJKUS

Vydání

Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2018, 0021-9533

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.517

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/18:00100849

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1242/jcs.202416

UT WoS

000424842400004

EID Scopus

2-s2.0-85041181109

Klíčová slova anglicky

A. thaliana rDNA; Replication; Nucleolus; Flow cytometry; Structured illumination microscopy

Štítky

cbia-web, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 3. 2019 09:06, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2'-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.

Návaznosti

GJ16-04166Y, projekt VaV

Název: Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis

Investor: Grantová agentura ČR, Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis

LH15189, projekt VaV

Název: Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana

LM2015062, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

DVOŘÁČKOVÁ, Martina; B. RAPOSO; Petr MATULA; J. FUCHS; V. SCHUBERT; Vratislav PEŠKA; B. DESVOYES; C. GUTIERREZ a Jiří FAJKUS. Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2018, roč. 131, č. 2, s. nestránkováno, 12 s. ISSN 0021-9533. Dostupné z: https://doi.org/10.1242/jcs.202416.

@article{1410141,
   author = {Dvořáčková, Martina and Raposo, B. and Matula, Petr and Fuchs, J. and Schubert, V. and Peška, Vratislav and Desvoyes, B. and Gutierrez, C. and Fajkus, Jiří},
   article_location = {Cambridge},
   article_number = {2},
   doi = {https://doi.org/10.1242/jcs.202416},
   keywords = {A. thaliana rDNA; Replication; Nucleolus; Flow cytometry; Structured illumination microscopy},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression},
   url = {http://jcs.biologists.org/content/131/2/jcs202416},
   volume = {131},
   year = {2018}
}

TY  - JOUR
ID  - 1410141
AU  - Dvořáčková, Martina - Raposo, B. - Matula, Petr - Fuchs, J. - Schubert, V. - Peška, Vratislav - Desvoyes, B. - Gutierrez, C. - Fajkus, Jiří
PY  - 2018
TI  - Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression
JF  - Journal of Cell Science
VL  - 131
IS  - 2
SP  - nestránkováno
EP  - nestránkováno
PB  - Company of Biologists Ltd.
SN  - 00219533
KW  - A. thaliana rDNA
KW  - Replication
KW  - Nucleolus
KW  - Flow cytometry
KW  - Structured illumination microscopy
UR  - http://jcs.biologists.org/content/131/2/jcs202416
L2  - http://jcs.biologists.org/content/131/2/jcs202416
N2  - Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2'-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.
ER  -

DVOŘÁČKOVÁ, Martina; B. RAPOSO; Petr MATULA; J. FUCHS; V. SCHUBERT; Vratislav PEŠKA; B. DESVOYES; C. GUTIERREZ a Jiří FAJKUS. Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2018, roč.~131, č.~2, s.~nestránkováno, 12 s. ISSN~0021-9533. Dostupné z: https://doi.org/10.1242/jcs.202416.

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