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@article{1410141,
author = {Dvořáčková, Martina and Raposo, B. and Matula, Petr and Fuchs, J. and Schubert, V. and Peška, Vratislav and Desvoyes, B. and Gutierrez, C. and Fajkus, Jiří},
article_location = {Cambridge},
article_number = {2},
doi = {http://dx.doi.org/10.1242/jcs.202416},
keywords = {A. thaliana rDNA; Replication; Nucleolus; Flow cytometry; Structured illumination microscopy},
language = {eng},
issn = {0021-9533},
journal = {Journal of Cell Science},
title = {Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression},
url = {http://jcs.biologists.org/content/131/2/jcs202416},
volume = {131},
year = {2018}
}
TY - JOUR
ID - 1410141
AU - Dvořáčková, Martina - Raposo, B. - Matula, Petr - Fuchs, J. - Schubert, V. - Peška, Vratislav - Desvoyes, B. - Gutierrez, C. - Fajkus, Jiří
PY - 2018
TI - Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression
JF - Journal of Cell Science
VL - 131
IS - 2
SP - nestránkováno
EP - nestránkováno
PB - Company of Biologists Ltd.
SN - 00219533
KW - A. thaliana rDNA
KW - Replication
KW - Nucleolus
KW - Flow cytometry
KW - Structured illumination microscopy
UR - http://jcs.biologists.org/content/131/2/jcs202416
L2 - http://jcs.biologists.org/content/131/2/jcs202416
N2 - Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2'-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.
ER -
DVOŘÁČKOVÁ, Martina, B. RAPOSO, Petr MATULA, J. FUCHS, V. SCHUBERT, Vratislav PEŠKA, B. DESVOYES, C. GUTIERREZ a Jiří FAJKUS. Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., roč.~131, č.~2, s.~nestránkováno, 12 s. ISSN~0021-9533. doi:10.1242/jcs.202416. 2018.
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