TOKAN, Viktor, Janka PUTEROVÁ, Matej LEXA a Eduard KEJNOVSKÝ. Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast. BMC Genomics, BioMed Central, 2018, roč. 19, 06 03 2018, s. 184-194. ISSN 1471-2164. doi:10.1186/s12864-018-4563-7.
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Základní údaje
Originální název Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast
Autoři TOKAN, Viktor (203 Česko, garant), Janka PUTEROVÁ (703 Slovensko), Matej LEXA (703 Slovensko, domácí) a Eduard KEJNOVSKÝ (203 Česko).
Vydání BMC Genomics, BioMed Central, 2018, 1471-2164.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10602 Biology , Evolutionary biology
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 3.501
Kód RIV RIV/00216224:14330/18:00100852
Organizační jednotka Fakulta informatiky
Doi http://dx.doi.org/10.1186/s12864-018-4563-7
UT WoS 000427128900005
Klíčová slova anglicky G4 motifs; Quadruplex DNA; Transposable elements; Maize LTR retrotransposons; Circular dichroism; NMM ligand
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: Ing. Matej Lexa, Ph.D., učo 31298. Změněno: 2. 10. 2018 15:06.
Anotace
Background Many studies have shown that guanine-rich DNA sequences form quadruplex structures (G4) in vitro but there is scarce evidence of guanine quadruplexes in vivo. The majority of potential quadruplex-forming sequences (PQS) are located in transposable elements (TEs), especially close to promoters within long terminal repeats of plant LTR retrotransposons. Results In order to test the potential effect of G4s on retrotransposon expression, we cloned the long terminal repeats of selected maize LTR retrotransposons upstream of the lacZ reporter gene and measured its transcription and translation in yeast. We found that G4s had an inhibitory effect on translation in vivo since “mutants” (where guanines were replaced by adenines in PQS) showed higher expression levels than wild-types. In parallel, we confirmed by circular dichroism measurements that the selected sequences can indeed adopt G4 conformation in vitro. Analysis of RNA-Seq of polyA RNA in maize seedlings grown in the presence of a G4-stabilizing ligand (NMM) showed both inhibitory as well as stimulatory effects on the transcription of LTR retrotransposons. Conclusions Our results demonstrate that quadruplex DNA located within long terminal repeats of LTR retrotransposons can be formed in vivo and that it plays a regulatory role in the LTR retrotransposon life-cycle, thus also affecting genome dynamics.
Návaznosti
GA15-02891S, projekt VaVNázev: Rostlinné transpozony a konformace DNA
Investor: Grantová agentura ČR, Standardní projekty
VytisknoutZobrazeno: 13. 8. 2020 03:04