TOKAN, Viktor, Janka PUTEROVÁ, Matej LEXA and Eduard KEJNOVSKÝ. Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast. BMC Genomics, BioMed Central, 2018, vol. 19, 06 03 2018, p. 184-194. ISSN 1471-2164. doi:10.1186/s12864-018-4563-7.
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Basic information
Original name Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast
Authors TOKAN, Viktor (203 Czechia, guarantor), Janka PUTEROVÁ (703 Slovakia), Matej LEXA (703 Slovakia, belonging to the institution) and Eduard KEJNOVSKÝ (203 Czechia).
Edition BMC Genomics, BioMed Central, 2018, 1471-2164.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10602 Biology , Evolutionary biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.501
RIV identification code RIV/00216224:14330/18:00100852
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1186/s12864-018-4563-7
UT WoS 000427128900005
Keywords in English G4 motifs; Quadruplex DNA; Transposable elements; Maize LTR retrotransposons; Circular dichroism; NMM ligand
Tags International impact, Reviewed
Changed by Changed by: Ing. Matej Lexa, Ph.D., učo 31298. Changed: 2/10/2018 15:06.
Abstract
Background Many studies have shown that guanine-rich DNA sequences form quadruplex structures (G4) in vitro but there is scarce evidence of guanine quadruplexes in vivo. The majority of potential quadruplex-forming sequences (PQS) are located in transposable elements (TEs), especially close to promoters within long terminal repeats of plant LTR retrotransposons. Results In order to test the potential effect of G4s on retrotransposon expression, we cloned the long terminal repeats of selected maize LTR retrotransposons upstream of the lacZ reporter gene and measured its transcription and translation in yeast. We found that G4s had an inhibitory effect on translation in vivo since “mutants” (where guanines were replaced by adenines in PQS) showed higher expression levels than wild-types. In parallel, we confirmed by circular dichroism measurements that the selected sequences can indeed adopt G4 conformation in vitro. Analysis of RNA-Seq of polyA RNA in maize seedlings grown in the presence of a G4-stabilizing ligand (NMM) showed both inhibitory as well as stimulatory effects on the transcription of LTR retrotransposons. Conclusions Our results demonstrate that quadruplex DNA located within long terminal repeats of LTR retrotransposons can be formed in vivo and that it plays a regulatory role in the LTR retrotransposon life-cycle, thus also affecting genome dynamics.
Links
GA15-02891S, research and development projectName: Rostlinné transpozony a konformace DNA
Investor: Czech Science Foundation, Standard Projects
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