Platinum nanoparticles induce damage to DNA and inhibit DNA
replication

J 2017

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

NEJDL, Lukas, Jiri KUDR, Amitava MOULICK, Dagmar HEGEROVA, Branislav RUTTKAY-NEDECKY et. al.

Basic information

Original name

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Authors

NEJDL, Lukas (203 Czech Republic), Jiri KUDR (203 Czech Republic), Amitava MOULICK (203 Czech Republic), Dagmar HEGEROVA (203 Czech Republic), Branislav RUTTKAY-NEDECKY (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, guarantor, belonging to the institution), Kristyna CIHALOVA (203 Czech Republic), Kristyna SMERKOVA (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Sona KRIZKOVA (203 Czech Republic), Marie NOVOTNA (203 Czech Republic), Pavel KOPEL (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)

Edition

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14110/17:00100237

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0180798

UT WoS

000405649600050

Keywords in English

Platinum nanoparticles; DNA

Abstract

V originále

Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage., on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.

Citovat

NEJDL, Lukas, Jiri KUDR, Amitava MOULICK, Dagmar HEGEROVA, Branislav RUTTKAY-NEDECKY, Jaromír GUMULEC, Kristyna CIHALOVA, Kristyna SMERKOVA, Simona DOSTALOVA, Sona KRIZKOVA, Marie NOVOTNA, Pavel KOPEL and Vojtech ADAM. Platinum nanoparticles induce damage to DNA and inhibit DNA replication. Plos one. San Francisco: Public Library of Science, 2017, vol. 12, No 7, p. 1-19. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180798.

@article{1410257,
   author = {Nejdl, Lukas and Kudr, Jiri and Moulick, Amitava and Hegerova, Dagmar and RuttkayandNedecky, Branislav and Gumulec, Jaromír and Cihalova, Kristyna and Smerkova, Kristyna and Dostalova, Simona and Krizkova, Sona and Novotna, Marie and Kopel, Pavel and Adam, Vojtech},
   article_location = {San Francisco},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0180798},
   keywords = {Platinum nanoparticles; DNA},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Platinum nanoparticles induce damage to DNA and inhibit DNA replication},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1410257
AU  - Nejdl, Lukas - Kudr, Jiri - Moulick, Amitava - Hegerova, Dagmar - Ruttkay-Nedecky, Branislav - Gumulec, Jaromír - Cihalova, Kristyna - Smerkova, Kristyna - Dostalova, Simona - Krizkova, Sona - Novotna, Marie - Kopel, Pavel - Adam, Vojtech
PY  - 2017
TI  - Platinum nanoparticles induce damage to DNA and inhibit DNA replication
JF  - Plos one
VL  - 12
IS  - 7
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - Platinum nanoparticles
KW  - DNA
N2  - Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage., on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.
ER  -

NEJDL, Lukas, Jiri KUDR, Amitava MOULICK, Dagmar HEGEROVA, Branislav RUTTKAY-NEDECKY, Jaromír GUMULEC, Kristyna CIHALOVA, Kristyna SMERKOVA, Simona DOSTALOVA, Sona KRIZKOVA, Marie NOVOTNA, Pavel KOPEL and Vojtech ADAM. Platinum nanoparticles induce damage to DNA and inhibit DNA replication. \textit{Plos one}. San Francisco: Public Library of Science, 2017, vol.~12, No~7, p.~1-19. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180798.

Detailed Information on Publication Record