NEJDL, Lukas, Jiri KUDR, Amitava MOULICK, Dagmar HEGEROVA, Branislav RUTTKAY-NEDECKY, Jaromír GUMULEC, Kristyna CIHALOVA, Kristyna SMERKOVA, Simona DOSTALOVA, Sona KRIZKOVA, Marie NOVOTNA, Pavel KOPEL and Vojtech ADAM. Platinum nanoparticles induce damage to DNA and inhibit DNA replication. Plos one. San Francisco: Public Library of Science, vol. 12, No 7, p. 1-19. ISSN 1932-6203. doi:10.1371/journal.pone.0180798. 2017.
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Basic information
Original name Platinum nanoparticles induce damage to DNA and inhibit DNA replication
Authors NEJDL, Lukas (203 Czech Republic), Jiri KUDR (203 Czech Republic), Amitava MOULICK (203 Czech Republic), Dagmar HEGEROVA (203 Czech Republic), Branislav RUTTKAY-NEDECKY (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, guarantor, belonging to the institution), Kristyna CIHALOVA (203 Czech Republic), Kristyna SMERKOVA (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Sona KRIZKOVA (203 Czech Republic), Marie NOVOTNA (203 Czech Republic), Pavel KOPEL (203 Czech Republic) and Vojtech ADAM (203 Czech Republic).
Edition Plos one, San Francisco, Public Library of Science, 2017, 1932-6203.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.766
RIV identification code RIV/00216224:14110/17:00100237
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1371/journal.pone.0180798
UT WoS 000405649600050
Keywords in English Platinum nanoparticles; DNA
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 8/3/2018 12:37.
Abstract
Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage., on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.
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