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@article{1410576, author = {Valuchová, Soňa and Fulneček, Jaroslav and Prokop, Zbyněk and StoltandBergner, Peggy and Janoušková, Eliška and Hofr, Ctirad and Říha, Karel}, article_location = {ROCKVILLE}, article_number = {6}, doi = {http://dx.doi.org/10.1105/tpc.17.00064}, keywords = {STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST}, language = {eng}, issn = {1040-4651}, journal = {Plant Cell}, title = {Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer}, url = {http://www.plantcell.org/content/29/6/1533}, volume = {29}, year = {2017} }
TY - JOUR ID - 1410576 AU - Valuchová, Soňa - Fulneček, Jaroslav - Prokop, Zbyněk - Stolt-Bergner, Peggy - Janoušková, Eliška - Hofr, Ctirad - Říha, Karel PY - 2017 TI - Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer JF - Plant Cell VL - 29 IS - 6 SP - 1533-1545 EP - 1533-1545 PB - AMER SOC PLANT BIOLOGISTS SN - 10404651 KW - STRAND BREAK REPAIR KW - INDUCED FLUORESCENCE ENHANCEMENT KW - SINGLE DNA END KW - MAMMALIAN TELOMERES KW - PROTEIN KW - LENGTH KW - COMPLEX KW - DAMAGE KW - FUSIONS KW - YEAST UR - http://www.plantcell.org/content/29/6/1533 L2 - http://www.plantcell.org/content/29/6/1533 N2 - Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries. ER -
VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR and Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, vol.~29, No~6, p.~1533-1545. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.17.00064.
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