Elasticity and tumorigenic characteristics of cells in a
monolayer after nanosecond pulsed electric field exposure

J 2017

Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure

STEUER, A., K. WENDE, Pavel BABICA and J. F. KOLB

Basic information

Original name

Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure

Authors

STEUER, A. (276 Germany), K. WENDE (276 Germany), Pavel BABICA (203 Czech Republic, guarantor, belonging to the institution) and J. F. KOLB (276 Germany)

Edition

European Biophysics Journal With Biophysics Letters, NEW YORK, SPRINGER, 2017, 0175-7571

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.935

RIV identification code

RIV/00216224:14310/17:00100310

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00249-017-1205-y

UT WoS

000407241700008

Keywords in English

nsPEF; Elasticity; Elastic modulus; AFM; Actin cytoskeleton; Anchorage-independent growth

Abstract

V originále

Nanosecond pulsed electric fields (nsPEFs) applied to cells can induce different biological effects depending on pulse duration and field strength. One known process is the induction of apoptosis whereby nsPEFs are currently investigated as a novel cancer therapy. Another and probably related change is the breakdown of the cytoskeleton. We investigated the elasticity of rat liver epithelial cells WB-F344 in a monolayer using atomic force microscopy (AFM) with respect to the potential of cells to undergo malignant transformation or to develop a potential to metastasize. We found that the elastic modulus of the cells decreased significantly within the first 8 min after treatment with 20 pulses of 100 ns and with a field strength of 20 kV/cm but was still higher than the elasticity of their tumorigenic counterpart WB-ras. AFM measurements and immunofluorescent staining showed that the cellular actin cytoskeleton became reorganized within 5 min. However, both a colony formation assay and a cell migration assay revealed no significant changes after nsPEF treatment, implying that cells seem not to adopt malignant characteristics associated with metastasis formation despite the induced transient changes to elasticity and cytoskeleton that can be observed for up to 1 h.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

STEUER, A., K. WENDE, Pavel BABICA and J. F. KOLB. Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure. European Biophysics Journal With Biophysics Letters. NEW YORK: SPRINGER, 2017, vol. 46, No 6, p. 567-580. ISSN 0175-7571. Available from: https://dx.doi.org/10.1007/s00249-017-1205-y.

@article{1410911,
   author = {Steuer, A. and Wende, K. and Babica, Pavel and Kolb, J. F.},
   article_location = {NEW YORK},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s00249-017-1205-y},
   keywords = {nsPEF; Elasticity; Elastic modulus; AFM; Actin cytoskeleton; Anchorage-independent growth},
   language = {eng},
   issn = {0175-7571},
   journal = {European Biophysics Journal With Biophysics Letters},
   title = {Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure},
   url = {https://link.springer.com/article/10.1007%2Fs00249-017-1205-y},
   volume = {46},
   year = {2017}
}

TY  - JOUR
ID  - 1410911
AU  - Steuer, A. - Wende, K. - Babica, Pavel - Kolb, J. F.
PY  - 2017
TI  - Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure
JF  - European Biophysics Journal With Biophysics Letters
VL  - 46
IS  - 6
SP  - 567-580
EP  - 567-580
PB  - SPRINGER
SN  - 01757571
KW  - nsPEF
KW  - Elasticity
KW  - Elastic modulus
KW  - AFM
KW  - Actin cytoskeleton
KW  - Anchorage-independent growth
UR  - https://link.springer.com/article/10.1007%2Fs00249-017-1205-y
L2  - https://link.springer.com/article/10.1007%2Fs00249-017-1205-y
N2  - Nanosecond pulsed electric fields (nsPEFs) applied to cells can induce different biological effects depending on pulse duration and field strength. One known process is the induction of apoptosis whereby nsPEFs are currently investigated as a novel cancer therapy. Another and probably related change is the breakdown of the cytoskeleton. We investigated the elasticity of rat liver epithelial cells WB-F344 in a monolayer using atomic force microscopy (AFM) with respect to the potential of cells to undergo malignant transformation or to develop a potential to metastasize. We found that the elastic modulus of the cells decreased significantly within the first 8 min after treatment with 20 pulses of 100 ns and with a field strength of 20 kV/cm but was still higher than the elasticity of their tumorigenic counterpart WB-ras. AFM measurements and immunofluorescent staining showed that the cellular actin cytoskeleton became reorganized within 5 min. However, both a colony formation assay and a cell migration assay revealed no significant changes after nsPEF treatment, implying that cells seem not to adopt malignant characteristics associated with metastasis formation despite the induced transient changes to elasticity and cytoskeleton that can be observed for up to 1 h.
ER  -

STEUER, A., K. WENDE, Pavel BABICA and J. F. KOLB. Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure. \textit{European Biophysics Journal With Biophysics Letters}. NEW YORK: SPRINGER, 2017, vol.~46, No~6, p.~567-580. ISSN~0175-7571. Available from: https://dx.doi.org/10.1007/s00249-017-1205-y.

Detailed Information on Publication Record