Other formats:
BibTeX
LaTeX
RIS
@article{1411035, author = {Shagin, D.A. and Shagina, I.A. and Zaretsky, A.R. and Barsova, E.V. and Kelmanson, I.V. and Lukyanov, S. and Chudakov, Dmitriy and Shugay, Mikhail}, article_location = {LONDON}, article_number = {JUN}, doi = {http://dx.doi.org/10.1038/s41598-017-02727-8}, keywords = {DRUG-RESISTANCE MUTATIONS; DNA-POLYMERASE; RARE MUTATIONS; FIDELITY; AMPLIFICATION; SPECIFICITY}, language = {eng}, issn = {2045-2322}, journal = {Scientific reports}, title = {A high-throughput assay for quantitative measurement of PCR errors}, url = {https://www.nature.com/articles/s41598-017-02727-8.pdf}, volume = {7}, year = {2017} }
TY - JOUR ID - 1411035 AU - Shagin, D.A. - Shagina, I.A. - Zaretsky, A.R. - Barsova, E.V. - Kelmanson, I.V. - Lukyanov, S. - Chudakov, Dmitriy - Shugay, Mikhail PY - 2017 TI - A high-throughput assay for quantitative measurement of PCR errors JF - Scientific reports VL - 7 IS - JUN SP - 2718 EP - 2718 PB - NATURE PUBLISHING GROUP SN - 20452322 KW - DRUG-RESISTANCE MUTATIONS KW - DNA-POLYMERASE KW - RARE MUTATIONS KW - FIDELITY KW - AMPLIFICATION KW - SPECIFICITY UR - https://www.nature.com/articles/s41598-017-02727-8.pdf L2 - https://www.nature.com/articles/s41598-017-02727-8.pdf N2 - The accuracy with which DNA polymerase can replicate a template DNA sequence is an extremely important property that can vary by an order of magnitude from one enzyme to another. The rate of nucleotide misincorporation is shaped by multiple factors, including PCR conditions and proofreading capabilities, and proper assessment of polymerase error rate is essential for a wide range of sensitive PCR-based assays. In this paper, we describe a method for studying polymerase errors with exceptional resolution, which combines unique molecular identifier tagging and high-throughput sequencing. Our protocol is less laborious than commonly-used methods, and is also scalable, robust and accurate. In a series of nine PCR assays, we have measured a range of polymerase accuracies that is in line with previous observations. However, we were also able to comprehensively describe individual errors introduced by each polymerase after either 20 PCR cycles or a linear amplification, revealing specific substitution preferences and the diversity of PCR error frequency profiles. We also demonstrate that the detected high-frequency PCR errors are highly recurrent and that the position in the template sequence and polymerase-specific substitution preferences are among the major factors influencing the observed PCR error rate. ER -
SHAGIN, D.A., I.A. SHAGINA, A.R. ZARETSKY, E.V. BARSOVA, I.V. KELMANSON, S. LUKYANOV, Dmitriy CHUDAKOV and Mikhail SHUGAY. A high-throughput assay for quantitative measurement of PCR errors. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2017, vol.~7, JUN, p.~2718-2728. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-02727-8.
|