| KOST, L.A., ES. NIKITIN, V.O. IVANOVA, U. SUNG, E.V. PUTINTSEVA, Dmitriy CHUDAKOV, P.M. BALABAN, K.A. LUKYANOV a A.M. BOGDANOV. Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 9, s. nestránkováno, 13 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0184225. |
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@article{1411055,
author = {Kost, L.A. and Nikitin, ES. and Ivanova, V.O. and Sung, U. and Putintseva, E.V. and Chudakov, Dmitriy and Balaban, P.M. and Lukyanov, K.A. and Bogdanov, A.M.},
article_location = {San Francisco},
article_number = {9},
doi = {http://dx.doi.org/10.1371/journal.pone.0184225},
keywords = {MEMBRANE VOLTAGE; LIVING CELLS; PROBE; COMPLEMENTATION; NEURONS; BRIGHT},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor},
url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184225},
volume = {12},
year = {2017}
}
TY - JOUR
ID - 1411055
AU - Kost, L.A. - Nikitin, ES. - Ivanova, V.O. - Sung, U. - Putintseva, E.V. - Chudakov, Dmitriy - Balaban, P.M. - Lukyanov, K.A. - Bogdanov, A.M.
PY - 2017
TI - Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor
JF - Plos one
VL - 12
IS - 9
SP - nestránkováno
EP - nestránkováno
PB - Public Library of Science
SN - 19326203
KW - MEMBRANE VOLTAGE
KW - LIVING CELLS
KW - PROBE
KW - COMPLEMENTATION
KW - NEURONS
KW - BRIGHT
UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184225
L2 - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184225
N2 - Visualization of electrical activity in living cells represents an important challenge in context of basic neurophysiological studies. Here we report a new voltage sensitive fluorescent indicator which response could be detected by fluorescence monitoring in a single red channel. To the best of our knowledge, this is the first fluorescent protein-based voltage sensor which uses insertion-into-circular permutant topology to provide an efficient interaction between sensitive and reporter domains. Its fluorescent core originates from red fluorescent protein (FP) FusionRed, which has optimal spectral characteristics to be used in whole body imaging techniques. Indicators using the same domain topology could become a new perspective for the FP-based voltage sensors that are traditionally based on Forster resonance energy transfer (FRET).
ER -
KOST, L.A., ES. NIKITIN, V.O. IVANOVA, U. SUNG, E.V. PUTINTSEVA, Dmitriy CHUDAKOV, P.M. BALABAN, K.A. LUKYANOV a A.M. BOGDANOV. Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~9, s.~nestránkováno, 13 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0184225.
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