Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF, Mária SALEH, D. EECKHOUT, S. EL-SHOWK, H.Y. LI, S.L. ZHONG, G. DE JAEGER, N.P. MONGAN, Jan HEJÁTKO, Y. HELARIUTTA and R.G. FRAY. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. New Phytologist. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 215, No 1, p. 157-172. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.14586.

Basic information

• Original name: Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
• Authors: RŮŽIČKA, Kamil (203 Czech Republic, belonging to the institution), M. ZHANG (156 China), A. CAMPILHO (620 Portugal), Z. BODI (826 United Kingdom of Great Britain and Northern Ireland), M. KASHIF (246 Finland), Mária SALEH (703 Slovakia, belonging to the institution), D. EECKHOUT (56 Belgium), S. EL-SHOWK (246 Finland), H.Y. LI (826 United Kingdom of Great Britain and Northern Ireland), S.L. ZHONG (826 United Kingdom of Great Britain and Northern Ireland), G. DE JAEGER (56 Belgium), N.P. MONGAN (826 United Kingdom of Great Britain and Northern Ireland), Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution), Y. HELARIUTTA (826 United Kingdom of Great Britain and Northern Ireland) and R.G. FRAY (826 United Kingdom of Great Britain and Northern Ireland).
• Edition: New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2017, 0028-646X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10611 Plant sciences, botany
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 7.433
• RIV identification code: RIV/00216224:14740/17:00095575
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1111/nph.14586
• UT WoS: 000402413100016
• Keywords in English: Arabidopsis; HAKAI; mRNA methylation; N6-adenosine methylation (m(6)A); protoxylem; VIRILIZER
• Tags: CF CELLIM, rivok
• Tags: International impact, Reviewed

Abstract

N6-adenosine methylation (m(6)A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6)A writer proteins in Arabidopsis thaliana. The components required for m(6)A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6)A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6)A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m(6)A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.

Links

• EE2.3.20.0189, research and development project: Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace
• GAP501/12/0934, research and development project: Name: Role sestřihu RNA v buněčném osudu protoxylému

Investor: Czech Science Foundation

• LM2015062, research and development project: Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

• LM2015091, research and development project: Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR