Detailed Information on Publication Record
2017
Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF et. al.Basic information
Original name
Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
Authors
RŮŽIČKA, Kamil (203 Czech Republic, belonging to the institution), M. ZHANG (156 China), A. CAMPILHO (620 Portugal), Z. BODI (826 United Kingdom of Great Britain and Northern Ireland), M. KASHIF (246 Finland), Mária SALEH (703 Slovakia, belonging to the institution), D. EECKHOUT (56 Belgium), S. EL-SHOWK (246 Finland), H.Y. LI (826 United Kingdom of Great Britain and Northern Ireland), S.L. ZHONG (826 United Kingdom of Great Britain and Northern Ireland), G. DE JAEGER (56 Belgium), N.P. MONGAN (826 United Kingdom of Great Britain and Northern Ireland), Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution), Y. HELARIUTTA (826 United Kingdom of Great Britain and Northern Ireland) and R.G. FRAY (826 United Kingdom of Great Britain and Northern Ireland)
Edition
New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2017, 0028-646X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 7.433
RIV identification code
RIV/00216224:14740/17:00095575
Organization unit
Central European Institute of Technology
UT WoS
000402413100016
Keywords in English
Arabidopsis; HAKAI; mRNA methylation; N6-adenosine methylation (m(6)A); protoxylem; VIRILIZER
Tags
International impact, Reviewed
Změněno: 23/3/2018 11:10, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
N6-adenosine methylation (m(6)A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6)A writer proteins in Arabidopsis thaliana. The components required for m(6)A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6)A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6)A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m(6)A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
Links
EE2.3.20.0189, research and development project |
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GAP501/12/0934, research and development project |
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LM2015062, research and development project |
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LM2015091, research and development project |
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LQ1601, research and development project |
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