RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF, Mária SALEH, D. EECKHOUT, S. EL-SHOWK, H.Y. LI, S.L. ZHONG, G. DE JAEGER, N.P. MONGAN, Jan HEJÁTKO, Y. HELARIUTTA and R.G. FRAY. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. New Phytologist. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 215, No 1, p. 157-172. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.14586. |
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@article{1411127, author = {Růžička, Kamil and Zhang, M. and Campilho, A. and Bodi, Z. and Kashif, M. and Saleh, Mária and Eeckhout, D. and ElandShowk, S. and Li, H.Y. and Zhong, S.L. and De Jaeger, G. and Mongan, N.P. and Hejátko, Jan and Helariutta, Y. and Fray, R.G.}, article_location = {HOBOKEN}, article_number = {1}, doi = {http://dx.doi.org/10.1111/nph.14586}, keywords = {Arabidopsis; HAKAI; mRNA methylation; N6-adenosine methylation (m(6)A); protoxylem; VIRILIZER}, language = {eng}, issn = {0028-646X}, journal = {New Phytologist}, title = {Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI}, url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf}, volume = {215}, year = {2017} }
TY - JOUR ID - 1411127 AU - Růžička, Kamil - Zhang, M. - Campilho, A. - Bodi, Z. - Kashif, M. - Saleh, Mária - Eeckhout, D. - El-Showk, S. - Li, H.Y. - Zhong, S.L. - De Jaeger, G. - Mongan, N.P. - Hejátko, Jan - Helariutta, Y. - Fray, R.G. PY - 2017 TI - Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI JF - New Phytologist VL - 215 IS - 1 SP - 157-172 EP - 157-172 PB - WILEY-BLACKWELL SN - 0028646X KW - Arabidopsis KW - HAKAI KW - mRNA methylation KW - N6-adenosine methylation (m(6)A) KW - protoxylem KW - VIRILIZER UR - http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf L2 - http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf N2 - N6-adenosine methylation (m(6)A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6)A writer proteins in Arabidopsis thaliana. The components required for m(6)A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6)A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6)A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m(6)A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences. ER -
RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF, Mária SALEH, D. EECKHOUT, S. EL-SHOWK, H.Y. LI, S.L. ZHONG, G. DE JAEGER, N.P. MONGAN, Jan HEJÁTKO, Y. HELARIUTTA and R.G. FRAY. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. \textit{New Phytologist}. HOBOKEN: WILEY-BLACKWELL, 2017, vol.~215, No~1, p.~157-172. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.14586.
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