Identification of factors required for m(6)A mRNA methylation
in Arabidopsis reveals a role for the conserved E3 ubiquitin
ligase HAKAI

J 2017

Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF et. al.

Basic information

Original name

Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI

Authors

RŮŽIČKA, Kamil (203 Czech Republic, belonging to the institution), M. ZHANG (156 China), A. CAMPILHO (620 Portugal), Z. BODI (826 United Kingdom of Great Britain and Northern Ireland), M. KASHIF (246 Finland), Mária SALEH (703 Slovakia, belonging to the institution), D. EECKHOUT (56 Belgium), S. EL-SHOWK (246 Finland), H.Y. LI (826 United Kingdom of Great Britain and Northern Ireland), S.L. ZHONG (826 United Kingdom of Great Britain and Northern Ireland), G. DE JAEGER (56 Belgium), N.P. MONGAN (826 United Kingdom of Great Britain and Northern Ireland), Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution), Y. HELARIUTTA (826 United Kingdom of Great Britain and Northern Ireland) and R.G. FRAY (826 United Kingdom of Great Britain and Northern Ireland)

Edition

New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2017, 0028-646X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.433

RIV identification code

RIV/00216224:14740/17:00095575

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/nph.14586

UT WoS

000402413100016

Keywords in English

Arabidopsis; HAKAI; mRNA methylation; N6-adenosine methylation (m(6)A); protoxylem; VIRILIZER

Abstract

V originále

N6-adenosine methylation (m(6)A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6)A writer proteins in Arabidopsis thaliana. The components required for m(6)A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6)A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6)A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m(6)A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.

Links

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GAP501/12/0934, research and development project

Name: Role sestřihu RNA v buněčném osudu protoxylému

Investor: Czech Science Foundation

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

LM2015091, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF, Mária SALEH, D. EECKHOUT, S. EL-SHOWK, H.Y. LI, S.L. ZHONG, G. DE JAEGER, N.P. MONGAN, Jan HEJÁTKO, Y. HELARIUTTA and R.G. FRAY. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. New Phytologist. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 215, No 1, p. 157-172. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.14586.

@article{1411127,
   author = {Růžička, Kamil and Zhang, M. and Campilho, A. and Bodi, Z. and Kashif, M. and Saleh, Mária and Eeckhout, D. and ElandShowk, S. and Li, H.Y. and Zhong, S.L. and De Jaeger, G. and Mongan, N.P. and Hejátko, Jan and Helariutta, Y. and Fray, R.G.},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/nph.14586},
   keywords = {Arabidopsis; HAKAI; mRNA methylation; N6-adenosine methylation (m(6)A); protoxylem; VIRILIZER},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf},
   volume = {215},
   year = {2017}
}

TY  - JOUR
ID  - 1411127
AU  - Růžička, Kamil - Zhang, M. - Campilho, A. - Bodi, Z. - Kashif, M. - Saleh, Mária - Eeckhout, D. - El-Showk, S. - Li, H.Y. - Zhong, S.L. - De Jaeger, G. - Mongan, N.P. - Hejátko, Jan - Helariutta, Y. - Fray, R.G.
PY  - 2017
TI  - Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
JF  - New Phytologist
VL  - 215
IS  - 1
SP  - 157-172
EP  - 157-172
PB  - WILEY-BLACKWELL
SN  - 0028646X
KW  - Arabidopsis
KW  - HAKAI
KW  - mRNA methylation
KW  - N6-adenosine methylation (m(6)A)
KW  - protoxylem
KW  - VIRILIZER
UR  - http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/nph.14586/pdf
N2  - N6-adenosine methylation (m(6)A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6)A writer proteins in Arabidopsis thaliana. The components required for m(6)A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6)A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6)A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m(6)A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
ER  -

RŮŽIČKA, Kamil, M. ZHANG, A. CAMPILHO, Z. BODI, M. KASHIF, Mária SALEH, D. EECKHOUT, S. EL-SHOWK, H.Y. LI, S.L. ZHONG, G. DE JAEGER, N.P. MONGAN, Jan HEJÁTKO, Y. HELARIUTTA and R.G. FRAY. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI. \textit{New Phytologist}. HOBOKEN: WILEY-BLACKWELL, 2017, vol.~215, No~1, p.~157-172. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.14586.

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