| DANISZOVA, K.,, O. MIKULA, Miloš MACHOLÁN, I. POSPISILOVA, B. Voslajerova BIMOVA and Z. HIADLOVSKA. Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus). GENERAL AND COMPARATIVE ENDOCRINOLOGY. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, vol. 252, OCT, p. 186-192. ISSN 0016-6480. Available from: https://dx.doi.org/10.1016/j.ygcen.2017.06.023. |
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@article{1411206,
author = {Daniszova, K., and Mikula, O. and Macholán, Miloš and Pospisilova, I. and Bimova, B. Voslajerova and Hiadlovska, Z.},
article_number = {OCT},
doi = {http://dx.doi.org/10.1016/j.ygcen.2017.06.023},
keywords = {ACTH challenge; Endocrine activity; Corticosterone; Hormone metabolites; Mouse; Noninvasive monitoring},
language = {eng},
issn = {0016-6480},
journal = {GENERAL AND COMPARATIVE ENDOCRINOLOGY},
title = {Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)},
volume = {252},
year = {2017}
}
TY - JOUR
ID - 1411206
AU - Daniszova, K., - Mikula, O. - Macholán, Miloš - Pospisilova, I. - Bimova, B. Voslajerova - Hiadlovska, Z.
PY - 2017
TI - Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)
JF - GENERAL AND COMPARATIVE ENDOCRINOLOGY
VL - 252
IS - OCT
SP - 186-192
EP - 186-192
PB - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN - 00166480
KW - ACTH challenge
KW - Endocrine activity
KW - Corticosterone
KW - Hormone metabolites
KW - Mouse
KW - Noninvasive monitoring
N2 - Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the "ACM challenge" test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid ("stress") hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments. (C) 2017 Elsevier Inc. All rights reserved.
ER -
DANISZOVA, K.,, O. MIKULA, Miloš MACHOLÁN, I. POSPISILOVA, B. Voslajerova BIMOVA and Z. HIADLOVSKA. Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus). \textit{GENERAL AND COMPARATIVE ENDOCRINOLOGY}. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, vol.~252, OCT, p.~186-192. ISSN~0016-6480. Available from: https://dx.doi.org/10.1016/j.ygcen.2017.06.023.
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