Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia

GRILLOVÁ, Linda, Ivo SEDLÁČEK, Gabriela PÁCHNIKOVÁ, Eva STAŇKOVÁ, Pavel ŠVEC, Pavla HOLOCHOVÁ, Lenka MICENKOVÁ, Juraj BOSÁK, Iva SLANINOVÁ and David ŠMAJS. Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia. JOURNAL OF VETERINARY MEDICAL SCIENCE. Tokyo: JAPAN SOC VET SCI, vol. 80, No 1, p. 138-146. ISSN 0916-7250. doi:10.1292/jvms.17-0492. 2018.

Basic information

• Original name: Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
• Authors: GRILLOVÁ, Linda (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution), Gabriela PÁCHNIKOVÁ (703 Slovakia, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution).
• Edition: JOURNAL OF VETERINARY MEDICAL SCIENCE, Tokyo, JAPAN SOC VET SCI, 2018, 0916-7250.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10601 Cell biology
• Country of publisher: Japan
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.910
• RIV identification code: RIV/00216224:14110/18:00100860
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1292/jvms.17-0492
• UT WoS: 000425785000023
• Keywords in English: Antarctica; bacteriocins; cytolethal distending toxin; Escherichia albertii
• Tags: 14110513, podil, rivok
• Tags: International impact, Reviewed

Abstract

Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.

Links

• GA16-21649S, research and development project: Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

• LM2015078, research and development project: Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

• LO1214, research and development project: Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

• ROZV/25/LF/2017, interní kód MU: Name: LF - Příspěvek na IP 2017

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects