Characterization of four Escherichia albertii isolates
collected from animals living in Antarctica and Patagonia

J 2018

Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia

GRILLOVÁ, Linda, Ivo SEDLÁČEK, Gabriela PÁCHNIKOVÁ, Eva STAŇKOVÁ, Pavel ŠVEC et. al.

Basic information

Original name

Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia

Authors

GRILLOVÁ, Linda (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution), Gabriela PÁCHNIKOVÁ (703 Slovakia, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF VETERINARY MEDICAL SCIENCE, Tokyo, JAPAN SOC VET SCI, 2018, 0916-7250

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Japan

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.910

RIV identification code

RIV/00216224:14110/18:00100860

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1292/jvms.17-0492

UT WoS

000425785000023

Keywords in English

Antarctica; bacteriocins; cytolethal distending toxin; Escherichia albertii

Abstract

V originále

Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.

Links

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

ROZV/25/LF/2017, interní kód MU

Name: LF - Příspěvek na IP 2017

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

GRILLOVÁ, Linda, Ivo SEDLÁČEK, Gabriela PÁCHNIKOVÁ, Eva STAŇKOVÁ, Pavel ŠVEC, Pavla HOLOCHOVÁ, Lenka MICENKOVÁ, Juraj BOSÁK, Iva SLANINOVÁ and David ŠMAJS. Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia. JOURNAL OF VETERINARY MEDICAL SCIENCE. Tokyo: JAPAN SOC VET SCI, 2018, vol. 80, No 1, p. 138-146. ISSN 0916-7250. Available from: https://dx.doi.org/10.1292/jvms.17-0492.

@article{1411209,
   author = {Grillová, Linda and Sedláček, Ivo and Páchniková, Gabriela and Staňková, Eva and Švec, Pavel and Holochová, Pavla and Micenková, Lenka and Bosák, Juraj and Slaninová, Iva and Šmajs, David},
   article_location = {Tokyo},
   article_number = {1},
   doi = {http://dx.doi.org/10.1292/jvms.17-0492},
   keywords = {Antarctica; bacteriocins; cytolethal distending toxin; Escherichia albertii},
   language = {eng},
   issn = {0916-7250},
   journal = {JOURNAL OF VETERINARY MEDICAL SCIENCE},
   title = {Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/29249728},
   volume = {80},
   year = {2018}
}

TY  - JOUR
ID  - 1411209
AU  - Grillová, Linda - Sedláček, Ivo - Páchniková, Gabriela - Staňková, Eva - Švec, Pavel - Holochová, Pavla - Micenková, Lenka - Bosák, Juraj - Slaninová, Iva - Šmajs, David
PY  - 2018
TI  - Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
JF  - JOURNAL OF VETERINARY MEDICAL SCIENCE
VL  - 80
IS  - 1
SP  - 138-146
EP  - 138-146
PB  - JAPAN SOC VET SCI
SN  - 09167250
KW  - Antarctica
KW  - bacteriocins
KW  - cytolethal distending toxin
KW  - Escherichia albertii
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29249728
N2  - Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.
ER  -

GRILLOVÁ, Linda, Ivo SEDLÁČEK, Gabriela PÁCHNIKOVÁ, Eva STAŇKOVÁ, Pavel ŠVEC, Pavla HOLOCHOVÁ, Lenka MICENKOVÁ, Juraj BOSÁK, Iva SLANINOVÁ and David ŠMAJS. Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia. \textit{JOURNAL OF VETERINARY MEDICAL SCIENCE}. Tokyo: JAPAN SOC VET SCI, 2018, vol.~80, No~1, p.~138-146. ISSN~0916-7250. Available from: https://dx.doi.org/10.1292/jvms.17-0492.

Detailed Information on Publication Record