Organic Electrochemical Transistor Microplate for Real-Time
Cell Culture Monitoring

J 2017

Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ et. al.

Basic information

Original name

Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

Authors

SALYK, Ota (203 Czech Republic, guarantor), Jan VÍTEČEK (203 Czech Republic, belonging to the institution), Lukáš OMASTA (203 Czech Republic), Eva ŠAFAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Stanislav STŘÍTESKÝ (203 Czech Republic), Martin VALA (203 Czech Republic) and Martin WEITER (203 Czech Republic)

Edition

Applied Sciences-Basel, BASEL, SWITZERLAND, MDPI AG, 2017, 2076-3417

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.689

RIV identification code

RIV/00216224:14310/17:00100375

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/app7100998

UT WoS

000414457800036

Keywords in English

OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture

Abstract

V originále

Human cell cultures provide a potentially powerful means for pharmacological and toxicological research. A microplate with a multielectrode array of 96 organic electrochemical transistors (OECTs) based on the semiconductive polymer poly(3,4-ethylenedioxythio-phene):poly(styrene sulfonic acid) PEDOT:PSS was developed and fabricated by the screen printing method. It consists of a microplate of a 12 × 8 chimney-well array with transistors on the bottom. The OECT is circular with a channel of 1.5 mm2 in the centre surrounded by the circular gate electrode. The device is designed for electrogenic cell monitoring. Simulations with the electrolyte revealed good electrical characteristics and indicated the setup information of the experimental conditions. A transconductance of g = 1.4 mS was achieved in the wide range of gate voltages Vgs = ±0.4 V when the drain potential Vds = -0.735 V was set and the long term relaxation was compensated for. The time constant 0.15 s limited by the channel-electrolyte charge electrical double layer (EDL) capacitance was measured. The device was tested on a 3T3 fibroblast cell culture and the sudden environmental changes were recorded. The living cells can be observed on the channel of the OECT and during electrical stimulation by gate voltage, as well as during the source current response.

Citovat

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ, Martin VALA and Martin WEITER. Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring. Applied Sciences-Basel. BASEL, SWITZERLAND: MDPI AG, 2017, vol. 7, No 10, p. nestránkováno, 10 pp. ISSN 2076-3417. Available from: https://dx.doi.org/10.3390/app7100998.

@article{1411252,
   author = {Salyk, Ota and Víteček, Jan and Omasta, Lukáš and Šafaříková, Eva and Stříteský, Stanislav and Vala, Martin and Weiter, Martin},
   article_location = {BASEL, SWITZERLAND},
   article_number = {10},
   doi = {http://dx.doi.org/10.3390/app7100998},
   keywords = {OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture},
   language = {eng},
   issn = {2076-3417},
   journal = {Applied Sciences-Basel},
   title = {Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring},
   url = {http://www.mdpi.com/2076-3417/7/10/998/htm},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1411252
AU  - Salyk, Ota - Víteček, Jan - Omasta, Lukáš - Šafaříková, Eva - Stříteský, Stanislav - Vala, Martin - Weiter, Martin
PY  - 2017
TI  - Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring
JF  - Applied Sciences-Basel
VL  - 7
IS  - 10
SP  - nestránkováno
EP  - nestránkováno
PB  - MDPI AG
SN  - 20763417
KW  - OECT
KW  - screen printing
KW  - organic electrochemical transistor
KW  - PEDOT:PSS
KW  - microplate
KW  - multi-electrode array
KW  - cell culture
UR  - http://www.mdpi.com/2076-3417/7/10/998/htm
L2  - http://www.mdpi.com/2076-3417/7/10/998/htm
N2  - Human cell cultures provide a potentially powerful means for pharmacological and toxicological research. A microplate with a multielectrode array of 96 organic electrochemical transistors (OECTs) based on the semiconductive polymer poly(3,4-ethylenedioxythio-phene):poly(styrene sulfonic acid) PEDOT:PSS was developed and fabricated by the screen printing method. It consists of a microplate of a 12 × 8 chimney-well array with transistors on the bottom. The OECT is circular with a channel of 1.5 mm2 in the centre surrounded by the circular gate electrode. The device is designed for electrogenic cell monitoring. Simulations with the electrolyte revealed good electrical characteristics and indicated the setup information of the experimental conditions. A transconductance of g = 1.4 mS was achieved in the wide range of gate voltages Vgs = ±0.4 V when the drain potential Vds = -0.735 V was set and the long term relaxation was compensated for. The time constant 0.15 s limited by the channel-electrolyte charge electrical double layer (EDL) capacitance was measured. The device was tested on a 3T3 fibroblast cell culture and the sudden environmental changes were recorded. The living cells can be observed on the channel of the OECT and during electrical stimulation by gate voltage, as well as during the source current response.
ER  -

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ, Martin VALA and Martin WEITER. Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring. \textit{Applied Sciences-Basel}. BASEL, SWITZERLAND: MDPI AG, 2017, vol.~7, No~10, p.~nestránkováno, 10 pp. ISSN~2076-3417. Available from: https://dx.doi.org/10.3390/app7100998.

Detailed Information on Publication Record