Extensive flagellar remodeling during the complex life cycle of
Paratrypanosoma, an early-branching trypanosomatid

J 2017

Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid

SKALICKÝ, Tomáš, E. DOBAKOVA, R.J. WHEELER, M. TESAROVA, P. FLEGONTOV et. al.

Basic information

Original name

Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid

Authors

SKALICKÝ, Tomáš (203 Czech Republic, guarantor, belonging to the institution), E. DOBAKOVA (203 Czech Republic), R.J. WHEELER (826 United Kingdom of Great Britain and Northern Ireland), M. TESAROVA (203 Czech Republic), P. FLEGONTOV (203 Czech Republic), D. JIRSOVA (56 Belgium), J. VOTYPKA (203 Czech Republic), V. YURCHENKO (203 Czech Republic), F.J. AYALA (840 United States of America) and J. LUKES (203 Czech Republic)

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2017, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.504

RIV identification code

RIV/00216224:14740/17:00100392

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1073/pnas.1712311114

UT WoS

000414127400070

Keywords in English

trypanosomatid; evolution; flagellar remodeling; haptomonads; cytostome

Abstract

V originále

Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features are conserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania-Wke flagellum attachment zone, and a Trypanosoma cruzi-like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.

Links

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SKALICKÝ, Tomáš, E. DOBAKOVA, R.J. WHEELER, M. TESAROVA, P. FLEGONTOV, D. JIRSOVA, J. VOTYPKA, V. YURCHENKO, F.J. AYALA and J. LUKES. Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2017, vol. 114, No 44, p. 11757-11762. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1712311114.

@article{1411367,
   author = {Skalický, Tomáš and Dobakova, E. and Wheeler, R.J. and Tesarova, M. and Flegontov, P. and Jirsova, D. and Votypka, J. and Yurchenko, V. and Ayala, F.J. and Lukes, J.},
   article_location = {WASHINGTON},
   article_number = {44},
   doi = {http://dx.doi.org/10.1073/pnas.1712311114},
   keywords = {trypanosomatid; evolution; flagellar remodeling; haptomonads; cytostome},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid},
   url = {http://www.pnas.org/content/114/44/11757},
   volume = {114},
   year = {2017}
}

TY  - JOUR
ID  - 1411367
AU  - Skalický, Tomáš - Dobakova, E. - Wheeler, R.J. - Tesarova, M. - Flegontov, P. - Jirsova, D. - Votypka, J. - Yurchenko, V. - Ayala, F.J. - Lukes, J.
PY  - 2017
TI  - Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 114
IS  - 44
SP  - 11757-11762
EP  - 11757-11762
PB  - National Academy of Sciences
SN  - 00278424
KW  - trypanosomatid
KW  - evolution
KW  - flagellar remodeling
KW  - haptomonads
KW  - cytostome
UR  - http://www.pnas.org/content/114/44/11757
L2  - http://www.pnas.org/content/114/44/11757
N2  - Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania. This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features are conserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania-Wke flagellum attachment zone, and a Trypanosoma cruzi-like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.
ER  -

SKALICKÝ, Tomáš, E. DOBAKOVA, R.J. WHEELER, M. TESAROVA, P. FLEGONTOV, D. JIRSOVA, J. VOTYPKA, V. YURCHENKO, F.J. AYALA and J. LUKES. Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2017, vol.~114, No~44, p.~11757-11762. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1712311114.

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