Detection of mycobacteria in the environment of the Moravian
Karst (Bull Rock Cave and the relevant water catchment area):
the impact of water sediment, earthworm castings and bat guano

J 2017

Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano

MODRÁ, Helena, Milan BARTOŠ, P. HŘIBOVÁ, Vít ULMANN, D. HUBELOVÁ et. al.

Basic information

Original name

Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano

Authors

MODRÁ, Helena (203 Czech Republic), Milan BARTOŠ (203 Czech Republic, belonging to the institution), P. HŘIBOVÁ (203 Czech Republic), Vít ULMANN (203 Czech Republic), D. HUBELOVÁ (203 Czech Republic), O. KONEČNÝ (203 Czech Republic), Milan GERŠL (203 Czech Republic), J. KUDĚLKA (203 Czech Republic), D. VOROS (203 Czech Republic), Ivo PAVLÍK (203 Czech Republic) and Petra KAUCKÁ

Edition

Veterinarni Medicina, Praha, Czech Academy of Agricultural Sciences, 2017, 0375-8427

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.434

RIV identification code

RIV/00216224:14310/17:00100394

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.17221/126/2016-VETMED

UT WoS

000396336300006

Keywords in English

bat faeces; environmentally derived mycobacteria; potentially pathogenic mycobacteria; ecology; geo-mycobacteriology; biospeleology; cave fauna; epidemiology

Abstract

V originále

The presence of mycobacteria was studied in Bull Rock Cave (“Byci skala”) and the water catch - ment area of Jedovnice Brook (“Jedovnicky potok”) using direct microscopy after Ziehl-Neelsen (ZN) staining, culture examination and molecular techniques. Mycobacteria were detected in 47.1% of a total of 68 samples. The mycobacterial genes hsp65 and dnaA were detected and sequenced in 37 (74.0%) out of the 50 cave environmental samples and in 10 (55.6%) out of the 18 samples of water catchment sediments. Nine species of slowly growing mycobacteria ( M. terrae , M. arupense , M. gordonae , M. lentiflavum , M. parascrofulaceum , M. parmense , M. sas - katchewanense , M. simiae and M. xenopi ) and two subsp. ( M. avium subsp. avium and M. avium subsp. hominis - suis ) were detected. Fourteen species of rapidly growing mycobacteria ( M. chelonae , M. chubuense , M. poriferae , M. flavescens , M. fortuitum , M. porcinum , M. rhodesiae , M. gilvum , M. goodii , M. peregrinum , M. mageritense , M. vanbaalenii , M. gadium and M. insubricum ) were detected. The highest mycobacterial presence was docu- mented by ZN staining and/or culture examinations in earthworm castings and bat guano (73.3% positivity out of the 15 samples) in the cave environment and in the water sediments collected under the outflow from the wastewater treatment plants (77.8% positivity out of nine samples). The highest total organic carbon (TOC) was detected in wooden material and earthworm castings with pH values between 5.0 and 7.7 in the cave environment and in water sediments collected under the outflow from the wastewater treatment plants with pH between 5.8 and 7.0. It could be concluded that the karst cave environment with its running surface water contaminated with different microorganisms or chemical substances creates favourable conditions not only for animals (especially earthworms) but also for mycobacteria. This fact is also demonstrated by the presence of these mycobacteria in the cave environment mainly in earthworm castings and bat guano.

Citovat

MODRÁ, Helena, Milan BARTOŠ, P. HŘIBOVÁ, Vít ULMANN, D. HUBELOVÁ, O. KONEČNÝ, Milan GERŠL, J. KUDĚLKA, D. VOROS, Ivo PAVLÍK and Petra KAUCKÁ. Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano. Veterinarni Medicina. Praha: Czech Academy of Agricultural Sciences, 2017, vol. 62, No 3, p. 153-168. ISSN 0375-8427. Available from: https://dx.doi.org/10.17221/126/2016-VETMED.

@article{1411392,
   author = {Modrá, Helena and Bartoš, Milan and Hřibová, P. and Ulmann, Vít and Hubelová, D. and Konečný, O. and Geršl, Milan and Kudělka, J. and Voros, D. and Pavlík, Ivo and Kaucká, Petra},
   article_location = {Praha},
   article_number = {3},
   doi = {http://dx.doi.org/10.17221/126/2016-VETMED},
   keywords = {bat faeces; environmentally derived mycobacteria; potentially pathogenic mycobacteria; ecology; geo-mycobacteriology; biospeleology; cave fauna; epidemiology},
   language = {eng},
   issn = {0375-8427},
   journal = {Veterinarni Medicina},
   title = {Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano},
   url = {http://vri.cz/docs/vetmed/62-3-153.pdf},
   volume = {62},
   year = {2017}
}

TY  - JOUR
ID  - 1411392
AU  - Modrá, Helena - Bartoš, Milan - Hřibová, P. - Ulmann, Vít - Hubelová, D. - Konečný, O. - Geršl, Milan - Kudělka, J. - Voros, D. - Pavlík, Ivo - Kaucká, Petra
PY  - 2017
TI  - Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano
JF  - Veterinarni Medicina
VL  - 62
IS  - 3
SP  - 153-168
EP  - 153-168
PB  - Czech Academy of Agricultural Sciences
SN  - 03758427
KW  - bat faeces
KW  - environmentally derived mycobacteria
KW  - potentially pathogenic mycobacteria
KW  - ecology
KW  - geo-mycobacteriology
KW  - biospeleology
KW  - cave fauna
KW  - epidemiology
UR  - http://vri.cz/docs/vetmed/62-3-153.pdf
L2  - http://vri.cz/docs/vetmed/62-3-153.pdf
N2  - The presence of mycobacteria was studied in Bull Rock Cave (“Byci skala”) and the water catch - ment area of Jedovnice Brook (“Jedovnicky potok”) using direct microscopy after Ziehl-Neelsen (ZN) staining, culture examination and molecular techniques. Mycobacteria were detected in 47.1% of a total of 68 samples. The mycobacterial genes hsp65 and dnaA were detected and sequenced in 37 (74.0%) out of the 50 cave environmental samples and in 10 (55.6%) out of the 18 samples of water catchment sediments. Nine species of slowly growing mycobacteria ( M. terrae , M. arupense , M. gordonae , M. lentiflavum , M. parascrofulaceum , M. parmense , M. sas - katchewanense , M. simiae and M. xenopi ) and two subsp. ( M. avium subsp. avium and M. avium subsp. hominis - suis ) were detected. Fourteen species of rapidly growing mycobacteria ( M. chelonae , M. chubuense , M. poriferae , M. flavescens , M. fortuitum , M. porcinum , M. rhodesiae , M. gilvum , M. goodii , M. peregrinum , M. mageritense , M. vanbaalenii , M. gadium and M. insubricum ) were detected. The highest mycobacterial presence was docu- mented by ZN staining and/or culture examinations in earthworm castings and bat guano (73.3% positivity out of the 15 samples) in the cave environment and in the water sediments collected under the outflow from the wastewater treatment plants (77.8% positivity out of nine samples). The highest total organic carbon (TOC) was detected in wooden material and earthworm castings with pH values between 5.0 and 7.7 in the cave environment and in water sediments collected under the outflow from the wastewater treatment plants with pH between 5.8 and 7.0. It could be concluded that the karst cave environment with its running surface water contaminated with different microorganisms or chemical substances creates favourable conditions not only for animals (especially earthworms) but also for mycobacteria. This fact is also demonstrated by the presence of these mycobacteria in the cave environment mainly in earthworm castings and bat guano.
ER  -

MODRÁ, Helena, Milan BARTOŠ, P. HŘIBOVÁ, Vít ULMANN, D. HUBELOVÁ, O. KONEČNÝ, Milan GERŠL, J. KUDĚLKA, D. VOROS, Ivo PAVLÍK and Petra KAUCKÁ. Detection of mycobacteria in the environment of the Moravian Karst (Bull Rock Cave and the relevant water catchment area): the impact of water sediment, earthworm castings and bat guano. \textit{Veterinarni Medicina}. Praha: Czech Academy of Agricultural Sciences, 2017, vol.~62, No~3, p.~153-168. ISSN~0375-8427. Available from: https://dx.doi.org/10.17221/126/2016-VETMED.

Detailed Information on Publication Record