FZD(10)-G alpha(13) signalling axis points to a role of FZD(10)
in CNS angiogenesis

J 2017

FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis

HOT, B., J. VALNOHOVA, E. ARTHOFER, K. SIMON, J. SHIN et. al.

Basic information

Original name

FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis

Authors

HOT, B. (752 Sweden), J. VALNOHOVA (203 Czech Republic), E. ARTHOFER (840 United States of America), K. SIMON (276 Germany), J. SHIN (752 Sweden), M. UHLEN (752 Sweden), E. KOSTENIS (276 Germany), J. MULDER (752 Sweden) and Gunnar SCHULTE (276 Germany, guarantor, belonging to the institution)

Edition

Cellular Signalling, NEW YORK, ELSEVIER SCIENCE INC, 2017, 0898-6568

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.487

RIV identification code

RIV/00216224:14310/17:00100422

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.cellsig.2017.01.023

UT WoS

000395953400010

Keywords in English

WNT; FZD; GPCR; Endothelial cell; Angiogenesis; GNA13; YAP/PAZ

Abstract

V originále

Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD(10) remains the most enigmatic. FZD10 shows homology to FZD(4) and FZD(9) and was previously implicated in both beta-catenin-dependent and-independent signalling. In normal tissue, FZD(10) levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD(10), as a receptor interacting with and signalling through the heterotrimeric G protein G alpha(13) but not G alpha(12), G alpha(i1,) G alpha(oA), G alpha(s), or G alpha(q). Stimulation with the FZD agonist WNT induced the dissociation of the G alpha(13) protein from FZD(10), and led to global G alpha(12/13)-dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD(10) mediates G alpha(12/13) activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD(10) in embryonic CNS endothelial cells at E11.5-E14.5. Given the well-known importance of G alpha(13) signalling for the development of the vascular system, the selective expression of FZD(10) in brain vascular endothelial cells points at a potential role of FZD(10)-G alpha(13) signalling in CNS angiogenesis. (C) 2017 Elsevier Inc. All rights reserved.

Links

EE2.3.20.0180, research and development project

Name: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny

Citovat

HOT, B., J. VALNOHOVA, E. ARTHOFER, K. SIMON, J. SHIN, M. UHLEN, E. KOSTENIS, J. MULDER and Gunnar SCHULTE. FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis. Cellular Signalling. NEW YORK: ELSEVIER SCIENCE INC, 2017, vol. 32, April, p. 93-103. ISSN 0898-6568. Available from: https://dx.doi.org/10.1016/j.cellsig.2017.01.023.

@article{1411805,
   author = {Hot, B. and Valnohova, J. and Arthofer, E. and Simon, K. and Shin, J. and Uhlen, M. and Kostenis, E. and Mulder, J. and Schulte, Gunnar},
   article_location = {NEW YORK},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.cellsig.2017.01.023},
   keywords = {WNT; FZD; GPCR; Endothelial cell; Angiogenesis; GNA13; YAP/PAZ},
   language = {eng},
   issn = {0898-6568},
   journal = {Cellular Signalling},
   title = {FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis},
   volume = {32},
   year = {2017}
}

TY  - JOUR
ID  - 1411805
AU  - Hot, B. - Valnohova, J. - Arthofer, E. - Simon, K. - Shin, J. - Uhlen, M. - Kostenis, E. - Mulder, J. - Schulte, Gunnar
PY  - 2017
TI  - FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis
JF  - Cellular Signalling
VL  - 32
IS  - April
SP  - 93-103
EP  - 93-103
PB  - ELSEVIER SCIENCE INC
SN  - 08986568
KW  - WNT
KW  - FZD
KW  - GPCR
KW  - Endothelial cell
KW  - Angiogenesis
KW  - GNA13
KW  - YAP/PAZ
N2  - Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD(10) remains the most enigmatic. FZD10 shows homology to FZD(4) and FZD(9) and was previously implicated in both beta-catenin-dependent and-independent signalling. In normal tissue, FZD(10) levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD(10), as a receptor interacting with and signalling through the heterotrimeric G protein G alpha(13) but not G alpha(12), G alpha(i1,) G alpha(oA), G alpha(s), or G alpha(q). Stimulation with the FZD agonist WNT induced the dissociation of the G alpha(13) protein from FZD(10), and led to global G alpha(12/13)-dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD(10) mediates G alpha(12/13) activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD(10) in embryonic CNS endothelial cells at E11.5-E14.5. Given the well-known importance of G alpha(13) signalling for the development of the vascular system, the selective expression of FZD(10) in brain vascular endothelial cells points at a potential role of FZD(10)-G alpha(13) signalling in CNS angiogenesis. (C) 2017 Elsevier Inc. All rights reserved.
ER  -

HOT, B., J. VALNOHOVA, E. ARTHOFER, K. SIMON, J. SHIN, M. UHLEN, E. KOSTENIS, J. MULDER and Gunnar SCHULTE. FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis. \textit{Cellular Signalling}. NEW YORK: ELSEVIER SCIENCE INC, 2017, vol.~32, April, p.~93-103. ISSN~0898-6568. Available from: https://dx.doi.org/10.1016/j.cellsig.2017.01.023.

Detailed Information on Publication Record