Structural and Surface Compatibility Study of Modified
Electrospun Poly(epsilon-caprolactone) (PCL) Composites for
Skin Tissue Engineering

J 2017

Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ and Sabu THOMAS

Basic information

Original name

Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

Authors

GHOSAL, Kajal (356 India), Anton MANAKHOV (643 Russian Federation, belonging to the institution), Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution) and Sabu THOMAS (356 India)

Edition

AAPS PHARMSCITECH, NEW YORK, SPRINGER, 2017, 1530-9932

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10305 Fluids and plasma physics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.666

RIV identification code

RIV/00216224:14740/17:00100438

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1208/s12249-016-0500-8

UT WoS

000392104200009

Keywords in English

compatibility study; composites; electrospinning; PCL; skin tissue engineering

Abstract

V originále

In this study, biodegradable poly(epsilon-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

Links

LD15150, research and development project

Name: Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

3SGA5652, interní kód MU

Name: Bioactive Nanofibers Prepared by Electrospinning and Plasma Technologies (Acronym: BioFibPlas)

Investor: South-Moravian Region, Incoming grants

Citovat

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ and Sabu THOMAS. Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering. AAPS PHARMSCITECH. NEW YORK: SPRINGER, 2017, vol. 18, No 1, p. 72-81. ISSN 1530-9932. Available from: https://dx.doi.org/10.1208/s12249-016-0500-8.

@article{1411915,
   author = {Ghosal, Kajal and Manakhov, Anton and Zajíčková, Lenka and Thomas, Sabu},
   article_location = {NEW YORK},
   article_number = {1},
   doi = {http://dx.doi.org/10.1208/s12249-016-0500-8},
   keywords = {compatibility study; composites; electrospinning; PCL; skin tissue engineering},
   language = {eng},
   issn = {1530-9932},
   journal = {AAPS PHARMSCITECH},
   title = {Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering},
   url = {https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1411915
AU  - Ghosal, Kajal - Manakhov, Anton - Zajíčková, Lenka - Thomas, Sabu
PY  - 2017
TI  - Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering
JF  - AAPS PHARMSCITECH
VL  - 18
IS  - 1
SP  - 72-81
EP  - 72-81
PB  - SPRINGER
SN  - 15309932
KW  - compatibility study
KW  - composites
KW  - electrospinning
KW  - PCL
KW  - skin tissue engineering
UR  - https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf
L2  - https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf
N2  - In this study, biodegradable poly(epsilon-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.
ER  -

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ and Sabu THOMAS. Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering. \textit{AAPS PHARMSCITECH}. NEW YORK: SPRINGER, 2017, vol.~18, No~1, p.~72-81. ISSN~1530-9932. Available from: https://dx.doi.org/10.1208/s12249-016-0500-8.

Detailed Information on Publication Record