BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive
Trafficking at the trans-Golgi Network/Early Endosome in
Arabidopsis thaliana

J 2017

BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana

KITAKURA, S., M. ADAMOWSKI, Y. MATSUURA, L. SANTUARI, H. KOUNO et. al.

Basic information

Original name

BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana

Authors

KITAKURA, S. (392 Japan), M. ADAMOWSKI (40 Austria), Y. MATSUURA (392 Japan), L. SANTUARI (528 Netherlands), H. KOUNO (392 Japan), K. ARIMA (392 Japan), C.S. HARDTKE (528 Netherlands), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution), T. KAKIMOTO (392 Japan) and H. TANAKA (392 Japan)

Edition

Plant and Cell Physiology, OXFORD, OXFORD UNIV PRESS, 2017, 0032-0781

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.059

RIV identification code

RIV/00216224:14740/17:00095594

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/pcp/pcx118

UT WoS

000413220400019

Keywords in English

Arabidopsis; ARF GEF; Auxin; Brefeldin A; PIN-FORMED1; trans-Golgi network

Abstract

V originále

Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to the fungal toxin brefeldin A (BFA), which is known to inhibit guanine nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been fully revealed. In a previous study, we identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. BEN3/BIG2 tagged with fluorescent proteins co-localized with markers for the TGN/early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA sensitive, and established BEN3/BIG2 as a crucial component of this BFA action at the level of the TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF, BEN1/MIN7. Taken together, our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

Citovat

KITAKURA, S., M. ADAMOWSKI, Y. MATSUURA, L. SANTUARI, H. KOUNO, K. ARIMA, C.S. HARDTKE, Jiří FRIML, T. KAKIMOTO and H. TANAKA. BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant and Cell Physiology. OXFORD: OXFORD UNIV PRESS, 2017, vol. 58, No 10, p. 1801-1811. ISSN 0032-0781. Available from: https://dx.doi.org/10.1093/pcp/pcx118.

@article{1412051,
   author = {Kitakura, S. and Adamowski, M. and Matsuura, Y. and Santuari, L. and Kouno, H. and Arima, K. and Hardtke, C.S. and Friml, Jiří and Kakimoto, T. and Tanaka, H.},
   article_location = {OXFORD},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/pcp/pcx118},
   keywords = {Arabidopsis; ARF GEF; Auxin; Brefeldin A; PIN-FORMED1; trans-Golgi network},
   language = {eng},
   issn = {0032-0781},
   journal = {Plant and Cell Physiology},
   title = {BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana},
   url = {https://academic.oup.com/pcp/article-abstract/58/10/1801/4086060?redirectedFrom=fulltext},
   volume = {58},
   year = {2017}
}

TY  - JOUR
ID  - 1412051
AU  - Kitakura, S. - Adamowski, M. - Matsuura, Y. - Santuari, L. - Kouno, H. - Arima, K. - Hardtke, C.S. - Friml, Jiří - Kakimoto, T. - Tanaka, H.
PY  - 2017
TI  - BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana
JF  - Plant and Cell Physiology
VL  - 58
IS  - 10
SP  - 1801-1811
EP  - 1801-1811
PB  - OXFORD UNIV PRESS
SN  - 00320781
KW  - Arabidopsis
KW  - ARF GEF
KW  - Auxin
KW  - Brefeldin A
KW  - PIN-FORMED1
KW  - trans-Golgi network
UR  - https://academic.oup.com/pcp/article-abstract/58/10/1801/4086060?redirectedFrom=fulltext
L2  - https://academic.oup.com/pcp/article-abstract/58/10/1801/4086060?redirectedFrom=fulltext
N2  - Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to the fungal toxin brefeldin A (BFA), which is known to inhibit guanine nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been fully revealed. In a previous study, we identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. BEN3/BIG2 tagged with fluorescent proteins co-localized with markers for the TGN/early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA sensitive, and established BEN3/BIG2 as a crucial component of this BFA action at the level of the TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF, BEN1/MIN7. Taken together, our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
ER  -

KITAKURA, S., M. ADAMOWSKI, Y. MATSUURA, L. SANTUARI, H. KOUNO, K. ARIMA, C.S. HARDTKE, Jiří FRIML, T. KAKIMOTO and H. TANAKA. BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. \textit{Plant and Cell Physiology}. OXFORD: OXFORD UNIV PRESS, 2017, vol.~58, No~10, p.~1801-1811. ISSN~0032-0781. Available from: https://dx.doi.org/10.1093/pcp/pcx118.

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